1887

Abstract

Summary

The transcription of temperate phage Mu throughout lytic development was analysed quantitatively by hybridization of pulse-labelled RNA to full-length Mu DNA and to plasmids that define Mu DNA segments covering the whole phage genome. The transcription rate (i.e. binding data corrected for the incorporation rate of the radioactive precursor, for the size of the DNA template, and for the number of phage genomes present in the bacterium at the time of analysis) revealed three defined phases of Mu transcription: early (0 to 9 min), intermediate (between 9 and the interval 14 to 17 min) and late (from the interval 14 to 17 min onward). The analysis also revealed that the region comprising the genes involved in phage morphogenesis was organized into two independent ‘late’ transcription units.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-2-385
1988-02-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/2/JV0690020385.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-2-385&mimeType=html&fmt=ahah

References

  1. BADE E. G. 1972; Asymmetric transcription of bacteriophage Mu-1. Journal of Virology 10:1205–1207
    [Google Scholar]
  2. BIRNBOIM H. C., DOLY J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. BOECKH C. 1980 Taq I-Restriktionskarte des temperenten E. coli-Phagen Mu. Clonierung und Charakterisierung relevanter Taq I-Fragmente in einem Plasmidvektor M.Sc. thesis, Universität Konstanz
    [Google Scholar]
  4. BOECKH C, BADE E. G., DELIUS H., REEVE J. 1986; Inhibition of bacterial segregation by early functions of phage Mu and association of replication protein B with the inner cell membrane. Molecular and General Genetics 202:461–466
    [Google Scholar]
  5. BOVRE K., SZYBALSKI W. 1971; Multistep DNA-RNA hybridization techniques. Methods in Enzymology 21:350–383
    [Google Scholar]
  6. BUKHARI A. I., AMBROSIO L. 1978; The invertible region of bacteriophage Mu DNA determines the adsorption properties of Mu particles. Nature, London 271:575–577
    [Google Scholar]
  7. CLAYTON R., SCHUMANN W., BADE E. G. 1981; In vitro insertions and deletions in the G segment of phage Mu DNA do not abolish the inversion process. Virology 109:267–280
    [Google Scholar]
  8. GIPHART-GASSLER M., REEVE J., VAN DE PUTTE P. 1981a; Polypeptides encoded by the early region of bacteriophage Mu synthesized in minicells of E. coli. Journal of Molecular Biology 145:165–191
    [Google Scholar]
  9. GIPHART-GASSLER M., WUFFELMAN C., REEVE J. 1981b; Structural polypeptides and products of late genes of bacteriophage Mu: characterization and functional aspects. Journal of Molecular Biology 145:139–163
    [Google Scholar]
  10. GOOSEN N., VAN DE PUTTE P. 1984; Regulation of Mu transposition. I. Localization of the presumed recognition sites for HimD and Ner functions controlling bacteriophage Mu transcription. Gene 30:41–46
    [Google Scholar]
  11. GOOSEN N., VAN HEUVEL M., MOOLENAAR G. F., VAN DE PUTTE P. 1984; Regulation of Mu transposition. II. The Escherichia coli HimD protein positively controls two repressor promoters and the early promoter of bacteriophage Mu. Gene 32:419–426
    [Google Scholar]
  12. GOOSEN T., GIPHART-GASSLER M., VAN DE PUTTE P. 1982; Bacteriophage Mu DNA replication is stimulated by non-essential early functions. Molecular and General Genetics 186:135–139
    [Google Scholar]
  13. HATTMAN S., IVES J. 1984; SI nuclease mapping of the phage Mu mom gene promoter: a model for the regulation of mom expression. Gene 29:185–198
    [Google Scholar]
  14. HATTMAN S., IVES J., MARGOLIN W., HOWE M. M. 1985; Regulation and expression of the bacteriophage Mu mom gene: mapping of the transactivation (Dad) function to the C region. Gene 39:71–76
    [Google Scholar]
  15. HEISIG P., KAHMANN R. 1986; The sequence and mom-transactivation function of the C gene of bacteriophage Mu. Gene 43:59–67
    [Google Scholar]
  16. KAHMANN R. 1983; Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu. Cold Spring Harbor Symposia on Quantitative Biology 47:639–646
    [Google Scholar]
  17. KAMP D., KAHMANN R., ZIPSER T. R., BROKER L. T., CHOW L. T. 1978; Inversion of the G segment of phage Mu controls phage infectivity. Nature, London 271:577–580
    [Google Scholar]
  18. KOURILSKY P., LEIDNER J., TREMBLAY G. Y. 1971; DNA-DNA hybridization on filters at low temperature in the presence of formamide or urea. Biochimie 53:1111–1114
    [Google Scholar]
  19. KRAUSE H. M., HIGGINS N. P. 1986; Positive and negative regulation of the Mu operator by Mu repressor and Escherichia coli integration host factor. Journal of Biological Chemistry 261:3744–3752
    [Google Scholar]
  20. KRAUSE H. M., ROTHWELL M. R., HIGGINS N. P. 1983; The early promoter of bacteriophage Mu: definition of the site of transcript initiation. Nucleic Acids Research 11:5483–5495
    [Google Scholar]
  21. MARGOLIN W., HOWE M. M. 1986; Localization and DNA sequence analysis of the C gene of bacteriophage Mu, the positive regulator of Mu late transcription. Nucleic Acids Research 14:4881–4897
    [Google Scholar]
  22. MARRS C. F. 1982 Transcription of bacteriophage Mu Ph.D. thesis, University of Wisconsin
    [Google Scholar]
  23. PAOLOZZI L., GHELARDINI P., LIEBART J. C, CAPOZZONI A., MARCHELLI C. 1980; Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase. Nucleic Acids Research 8:5859–5874
    [Google Scholar]
  24. PLASTERK R. H. A., BRINKMAN A., VAN DE PUTTE P. 1983; DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination systems. Proceedings of the National Academy of Sciences, U.S.A 80:5355–5358
    [Google Scholar]
  25. PLASTERK R. H. A., VOLLERING M., BRINKMAN A., VAN DE PUTTE P. 1984; Analysis of the methylation-regulated Mu mom transcript. Cell 36:189–196
    [Google Scholar]
  26. PRIESS H., KAMP D., KAHMANN R., BRäUER B., DELIUS H. 1982; Nucleotide sequence of the immunity region of bacteriophage Mu. Molecular and General Genetics 186:315–321
    [Google Scholar]
  27. SCHRöDER W., BADE E. G., DELIUS H. 1974; Participation of E. coli DNA in the replication of temperate bacteriophage Mu-1. Virology 60:534–542
    [Google Scholar]
  28. SCHUMANN W. 1979; Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids. Molecular and General Genetics 174:221–224
    [Google Scholar]
  29. SCHUMANN W., BADE E. G. 1979; In vitro constructed plasmids containing both ends of bacteriophage Mu DNA express phage functions. Molecular and General Genetics 169:97–105
    [Google Scholar]
  30. STAUFENBIEL M., SCHUMANN W. 1983; Cloning and characterization of restriction fragments of phage Mu DNA. FEMS Microbiology Letters 20:369–374
    [Google Scholar]
  31. SYMONDS N., COELHO A. 1978; Role of the G segment in the growth of phage Mu. Nature, London 271:573–574
    [Google Scholar]
  32. TOUSSAINT A. 1976; The DNA modification function of temperate phage Mu-1. Virology 70:17–27
    [Google Scholar]
  33. TOUSSAINT A., RéSIBOIS A. 1983 Phage Mu: transposition as a life-style. Mobile Genetic Elements105–158 Edited by Shapiro J. A. New York: Academic Press;
    [Google Scholar]
  34. VAN DE PUTTE P., GIPHART-GASSLER M., GOOSEN N., GOOSEN T., VAN LEERDAM E. 1980; Regulation of integration and replication functions of bacteriophage Mu. Cold Spring Harbor Symposia on Quantitative Biology 45:347–353
    [Google Scholar]
  35. VAN MEETEREN A. 1980 Transcription of bacteriophage Mu Ph.D. thesis, Rijksuniversiteit, Leiden
    [Google Scholar]
  36. VAN MEETEREN A., GIPHART-GASSLER M., VAN DE PUTTE P. 1980; Transcription of bacteriophage Mu. II. Transcription of the repressor gene. Molecular and General Genetics 179:185–190
    [Google Scholar]
  37. WUFFELMAN C., VAN DE PUTTE P. 1974; Transcription of bacteriophage Mu. An analysis of the transcription pattern in the early phase of phage development. Molecular and General Genetics 135:327–337
    [Google Scholar]
  38. WUFFELMAN C, GASSLER M., STEVENS W. F., VAN DE PUTTE P. 1974; On the control of transcription of bacteriophage Mu. Molecular and General Genetics 131:85–96
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-2-385
Loading
/content/journal/jgv/10.1099/0022-1317-69-2-385
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error