1887

Abstract

Summary

A broadly cross-reactive monoclonal antibody directed against papillomavirus, coupled to immunoaffinity columns, was used to isolate bovine papillomavirus type 1 (BPV-1) and human papillomavirus type 1 (HPV-1) structural polypeptides from homogenates of productively infected cells. One of the polypeptides isolated from bovine fibropapillomas appeared to be the BPV-1 major capsid protein since it had a mol. wt. of 54K and was reactive by Western blots with papillomavirus genus- and BPV-1 type-specific rabbit antibodies as well as monoclonal antibodies cross-reactive with BPV-l/BPV-2 and BPV-l/deer PV. A polypeptide from human plantar warts similarly appeared to be the major capsid component since it also had a mass of 54K to 55K and reacted with papillomavirus genus- and HPV type-specific rabbit antibodies. By using this technique structural viral polypeptides from papillomavirus-induced lesions containing readily detectable viral structural antigens but relatively few virus particles, such as seen with mucosotropic HP Vs can now be isolated for mapping of virus-specific epitopes.

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1987-07-01
2024-04-26
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References

  1. Butel J. 1972; Studies with human papillomavirus modeled after known papovavirus systems. Journal of the National Cancer Institute 48:285–289
    [Google Scholar]
  2. Coggin J. R.Jr Zur Hausen H. 1977; Workshop on papillomavirus and cancer. Cancer Research 39:545–546
    [Google Scholar]
  3. Crum C. P., Ikenberg H., Richart R. M., Gissmann L. 1984; Human papillomavirus type 16 and early cervical neoplasia. New England Journal of Medicine 310:880–884
    [Google Scholar]
  4. Favre M., Breitburd F., Croissant O., Orth O. 1975; Structural polypeptides of rabbit, bovine, and human papillomaviruses. Journal of Virology 15:1239–1247
    [Google Scholar]
  5. Gissmann L. 1984; Papillomaviruses and their association to cancer in animals and man. Cancer Survey 3:161–181
    [Google Scholar]
  6. Gissmann L., Pfister H., Zur Hausen H. 1977; Human papillomavirus (HPV): characterization of four different isolates. Virology 76:569–580
    [Google Scholar]
  7. Grussendorf-Cohen E. I., Gissmann L., Holters J. 1983; Correlation between content of viral DNA and evidence of mature viral particles in HPV1, HPV4 and HPV6-induced virus acanthoma. Journal of Investigative Dermatology 81:511–513
    [Google Scholar]
  8. Hancock K., Tsang V. 1983; India ink staining of proteins on nitrocellulose paper. Analytical Biochemistry 133:157–162
    [Google Scholar]
  9. Heilman C. A., Law M-F., Israel M. A., Howley P. A. 1980; Cloning of human papilloma virus genomic DNAs and analysis of homologous polynucleotide sequences. Journal of Virology 36:395–407
    [Google Scholar]
  10. Howley P. M. 1982; The human papillomaviruses. Archives of Pathology and Laboratory Medicine 106:429–432
    [Google Scholar]
  11. Jenson A. B., Rosenthal J. R., Olson C., Pass F., Lancaster W. D., Shah K. 1980; Immunological relatedness of papillomaviruses from different species. Journal of the National Cancer Institute 64:495–500
    [Google Scholar]
  12. Jenson A. B., Sommer S., Payling-Wright C., Pass F., Link C.Jr Lancaster W. D. 1982; Human papillomaviruses: frequency and distribution in plantar and common warts. Laboratory Investigation 47:491–497
    [Google Scholar]
  13. Jenson A. B., Kurman R. J., Lancaster W. D. 1984; Human papillomaviruses. In Textbook of Human Virology pp. 951–968 Belshe R. Edited by Massachusetts: PSG;
    [Google Scholar]
  14. Jenson A. B., Lancaster W. D., Kurman R. J. 1986; The cervix. In Pathology of Incipient Neoplasia pp. 249–263 Albores-Saavedra D. Henson. Edited by Philadelphia: W. B. Saunders;
    [Google Scholar]
  15. Lancaster W. D., Jenson A. B. 1981; Evidence of papillomavirus genus-specific antigens and DNA in laryngeal papilloma. Intervirology 15:204–212
    [Google Scholar]
  16. Lancaster W. D., Olson C. 1978; Demonstration of two distinct classes of bovine papilloma virus. Virology 79:371–379
    [Google Scholar]
  17. Lancaster W. D., Kurman R. J., Jenson A. B. 1986; Papillomavirus in anogenital neoplasms. In Molecular Analysis and Diagnosis of Malignancy pp. 153–183 Londerer O., Weetall O. Edited by New Jersey: Humana Press;
    [Google Scholar]
  18. Loring T., Ikenberg H., Becker J., Gissmann L., Hoepfer I., Zur Hausen H. 1985; Analysis of oral papillomas, leukoplakias, and invasive carcinomas for human papillomavirus type related DNA. Journal of Investigative Dermatology 84:417–420
    [Google Scholar]
  19. Nakai Y., Lancaster W. D., Jenson A. B. 1986; Monoclonal antibodies to genus- and type-specific papillomavirus structural antigens. Intervirology 25:30–37
    [Google Scholar]
  20. Orth G, Favre M. 1986; Human papillomaviruses: biochemical and biologic properties. Clinics in Dermatology 3(4):27–42
    [Google Scholar]
  21. Orth G., Breitburd F., Favre M. 1978; Evidence for antigenic determinants shared by the structural polypeptides of (Shope) rabbit papillomavirus and human papillomavirus type 1. Virology 91:243–255
    [Google Scholar]
  22. Pfister H. 1983; Biology and biochemistry of papillomaviruses. Review of Physiology, Biochemistry and Pharmacology 99:111–181
    [Google Scholar]
  23. Roseto A., Pothier P., Guillemin M-C., Peries J., Breitburd F., Bonneaud N., Orth G. 1984; Monoclonal antibodies to the major capsid protein of human papillomavirus type 1. Journal of General Virology 65:1319–1324
    [Google Scholar]
  24. Rowson K. E. K., Mahy B. W. J. 1967; Human papova (wart) virus. Bacteriological Reviews 31:110–131
    [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  26. Voller A., Bidwell D., Bartlett A. 1976; Enzyme immunoassays in diagnostic medicine. Bulletin of the World Health Organization 53:55–64
    [Google Scholar]
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