%0 Journal Article %A Nakai, Yutaka %A Lancaster, Wayne D. %A Jenson, A. Bennett %T Purification of Papillomavirus Structural Polypeptides from Papillomas by Immunoaffinity Chromatography %D 1987 %J Journal of General Virology, %V 68 %N 7 %P 1891-1896 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-68-7-1891 %K BPV-1 %K capsid protein %K monoclonal antibodies %I Microbiology Society, %X Summary A broadly cross-reactive monoclonal antibody directed against papillomavirus, coupled to immunoaffinity columns, was used to isolate bovine papillomavirus type 1 (BPV-1) and human papillomavirus type 1 (HPV-1) structural polypeptides from homogenates of productively infected cells. One of the polypeptides isolated from bovine fibropapillomas appeared to be the BPV-1 major capsid protein since it had a mol. wt. of 54K and was reactive by Western blots with papillomavirus genus- and BPV-1 type-specific rabbit antibodies as well as monoclonal antibodies cross-reactive with BPV-l/BPV-2 and BPV-l/deer PV. A polypeptide from human plantar warts similarly appeared to be the major capsid component since it also had a mass of 54K to 55K and reacted with papillomavirus genus- and HPV type-specific rabbit antibodies. By using this technique structural viral polypeptides from papillomavirus-induced lesions containing readily detectable viral structural antigens but relatively few virus particles, such as seen with mucosotropic HP Vs can now be isolated for mapping of virus-specific epitopes. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-7-1891