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1887

Journal of General Virology header logo Journal of General Virology

Volume 68, Issue 6

Nuclear DNA-binding Proteins Determined by the Epstein-Barr Virus-related Simian Lymphotropic Herpesviruses and No Access

Abstract

Summary

Nuclear DNA-binding proteins were extracted from lymphoblastoid cell lines transformed with Epstein-Barr virus (EBV) or with the related lymphotropic herpesviruses of gorilla (), chimpanzee (), baboon () or orang-utan (). They were immunoblotted with the sera of all four simian species in comparison with EBV antibody-positive human sera. Eight nuclear proteins were identified, and were designated GONA-1 and GONA-2 for -determined nuclear antigens, PANA-1A, PANA-1B and PANA-2 for , PONA-2 for and HUPNA-1 and HUPNA-2 for .papio-determined nuclear antigens. One of two tested HUPNA-2-positive baboon sera and one PONA-2-positive orang-utan serum also reacted with EBNA-2 in EBV-transformed cells. A human serum that contained antibodies to all five EBNA proteins cross-reacted only with PANA-2 and PONA-2. Monospecific anti-peptide antibodies against EBNA-2, type A, also reacted with PONA-2, but not with the other simian nuclear antigens. The data provide evidence that EBV-like simian lymphotropic herpesviruses induce EBNA-like nuclear antigens and that EBNA-2 and some simian EBNA-related proteins contain an epitope that has been conserved during the evolution of the EBV family of viruses.

  • Received:
  • Accepted:
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Keyword(s): herpesvirus and simian/nuclear proteins/EBV
© The Journal of General Virology 1987
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1987-06-01
2025-03-15
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References

  1. Alberts B., Herrick G. 1971; DNA cellulose chromatography. Methods in Enzymology 21:198–217
     [Google Scholar]
  2. Dambaugh T., Hennessy K., Chamnankit L., Kieff E. 1984; The U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2. Proceedings of the National Academy of Sciences U.S.A.: 817632–7636
     [Google Scholar]
  3. Dillner J., Sternas L., Kallin B., Alexander H., Ehlin-Henriksson B., Jornvall H., Klein G., Lerner R. 1984; Antibodies against a synthetic peptide identify the Epstein-Barr virus determined nuclear antigen. Proceedings of the National Academy of Sciences U.S.A.: 814652–4656
     [Google Scholar]
  4. Dillner J., Kallin B., Klein G., Jornvall H., Alexander H., Lerner R. 1985; Antibodies against synthetic peptides react with the second Epstein-Barr virus associated nuclear antigen. EMBO Journal 4:1813–1818
     [Google Scholar]
  5. Dillner J., Kallin B., Ehlin-Henriksson B., Rymo L., Henle W. 1986a; The Epstein- Barr virus determined nuclear antigen is composed of at least three different antigens. International Journal of Cancer 37:195–200
     [Google Scholar]
  6. Dillner J., Kallin B., Alexander H., Ernberg I., Uno M., Ono Y., Klein G., Lerner R. A. 1986b; An Epstein-Barr virus nuclear antigen (EBNA-5) partly encoded by the transformation-associated Bam WYH region: preferential expression in lymphoblastoid cell lines. Proceedings of the National Academy of Sciences U.S.A.: 836641–6645
     [Google Scholar]
  7. Ernberg I., Kallin B. 1984; Epstein-Barr virus and its association with human malignant diseases. Cancer Surveys 3:51–89
     [Google Scholar]
  8. Falk L., Deinhardt F., Nonoyama M., Wolfe L.G, Lapin B., Yakovleva L., Agrba V., Henle G. 1976; Properties of a baboon lymphotropic herpesvirus related to Epstein-Barr virus. International Journal of Cancer 18:798–807
     [Google Scholar]
  9. Gerber P., Prichett G. R. F., Kieff E. D. 1976; Antigens and DNA of a chimpanzee agent related to Epstein- Barr virus. Journal of Virology 19:1090–1099
     [Google Scholar]
  10. Gerber P., Kalter S. S., Schidlovsky G., Peterson W. D., Daniel M. D. 1977; Biological and antigenic characteristics of Epstein-Barr virus-related herpesviruses of chimpanzees and baboons. International Journal of Cancer 20:448–459
     [Google Scholar]
  11. Hearing J. C., Nicolas J. C., Levine A. J. 1984; Identification of Epstein-Barr virus sequences that encode a nuclear antigen expressed in latently infected lymphocytes. Proceedings of the National Academy of Sciences U.S.A.: 814373–4377
     [Google Scholar]
  12. Heller M. 1981; Colinearity between the DNAs of Epstein-Barr virus and Herpesvirus papio. Journal of Virology 37:821–826
     [Google Scholar]
  13. Heller M., Gerber P. 1981; Herpesvirus papio DNA is similar in organization to Epstein-Barr virus DNA. Journal of Virology 37:698–709
     [Google Scholar]
  14. Heller M., Van Santen V., Kieff E. 1982a; Simple repeat sequence in Epstein-Barr virus DNA is transcribed in latent and productive infections. Journal of Virology 44:311–320
     [Google Scholar]
  15. Heller M., Gerber P., Kieff E. 1982b; DNA of Herpesvirus pan, a third member of the Epstein-Barr virus- Herpesvirus papio group. Journal of Virology 41:931–939
     [Google Scholar]
  16. Heller M., Henderson A., Kieff E. 1982c; Repeat array in Epstein-Barr virus DNA is related to cell DNA sequences interspersed on human chromosomes. Proceedings of the National Academy of Sciences U.S.A.: 79:5916–5920
     [Google Scholar]
  17. Hennessy K., Kieff E. 1983; One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain. Proceedings of the National Academy of Sciences U.S.A.: 805665–5669
     [Google Scholar]
  18. Hennessy K., Kieff E. 1985; A second nuclear protein is encoded by Epstein-Bar virus in latent infection. Science 227:1238–1240
     [Google Scholar]
  19. Hennessy K., Fennewald S., Kieff E. 1985; A third viral nuclear protein in lymphoblasts immortalized by Epstein-Barr virus. Proceedings of the National Academy of Sciences U.S.A.: 825944–5948
     [Google Scholar]
  20. Kallin B., Dillner J., Ernberg I., Ehlin-Henriksson B., Rosen A., Henle W., Henle G., Klein G. 1986; Four virally determined nuclear antigens are expressed in Epstein-Barr virus-transformed cells. Proceedings of the National Academy of Sciences U.S.A.: 831499–1503
     [Google Scholar]
  21. Lee Y. S., Nonoyama M., Rabin H. 1981; Colinear relationships of Herpesvirus papio DNA to Epstein-Barr virus DNA. Virology 110:248–252
     [Google Scholar]
  22. Neubauer R. H., Rabin H., Strnad B. C., Nonoyama M., Nelson-Rees W.A. 1979; Establishment of a lymphoblastoid cell line and isolation of an Epstein-Barr (EBV)-related virus of gorilla origin. Journal of Virology 31:845–848
     [Google Scholar]
  23. Ohno S., Luka J., Falk L. A., Klein G. 1977; Detection of a nuclear, EBNA type antigen in apparently EBNA- negative Herpesvirus papio (HVP)-transformed lymphoid lines by the acid-fixed nuclear binding technique. International Journal of Cancer 20:941–946
     [Google Scholar]
  24. Ohno S., Luka J., Falk L. A., Klein G. 1978; Serological reactivities of human and baboon sera against EBNA and Herpesvirus papio-determined nuclear antigen (HUPNA). European Journal of Cancer 14:955–960
     [Google Scholar]
  25. Rabin H., Neubauer R. H., Hopkins R. F., Dzhikidze E. K., Shevtsova Z. V., Lapin B. A. 1977; Transforming activity and antigenicity of an Epstein-Barr (EBV)-like virus from lymphoblastoid cell lines of baboons with lymphoid disease. Intervirology 8:240–249
     [Google Scholar]
  26. Rabin H., Neubauer R. H., Hopkins R. F., Nonoyama M. 1978; Further characterization of a herpesviruspositive orang-utan cell line and comparative aspects of in vitro transformation with lymphotropic Old World primate herpesviruses. International Journal of Cancer 21:762–767
     [Google Scholar]
  27. Rabin H., Strnad B.C, Brown A. M., Hopkins R. F., Mazur R. A. 1980; Comparisons of nuclear antigens of Epstein-Barr virus (EBV) and EBV-like simian viruses. Journal of Virology 48:265–272
     [Google Scholar]
  28. Ragona G., Ernberg I., Klein G. 1980; Induction and biological characterization of the Epstein-Barr virus (EBV) carried by the Jijoye lymphoma line. Virology 101:553–557
     [Google Scholar]
  29. Rasheed S., Rongey R. W., Bruszweski J., Nelson-Rees W. A., Rabin H., Neubauer R. H., Esra G., Gardner M. B. 1977; Establishment of a cell line with associated Epstein-Barr-like virus from a leukemic orang-utan. Science 198:407–409
     [Google Scholar]
  30. Rawlins D. R., Milman G., Hayward S. D., Hayward G. S. 1985; Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region. Cell 42:859–868
     [Google Scholar]
  31. Skare J., Farley J., Strominger J. L., Fresen K. O., Cho M. S., Zurhausen H. 1985; Transformation by Epstein-Barr virus requires DNA sequences in the region of BamHl fragments Y and H. Journal of Virology 55:286–297
     [Google Scholar]
  32. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences U.S.A.: 764350–4354
     [Google Scholar]
  33. Van Santen V., Cheung A., Kieff E. 1981; Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line. Proceedings of the National Academy of Sciences U.S.A.: 781930–1934
     [Google Scholar]
  34. Yates J., Warren N., Reisman D., Sugden B. 1984; A cis acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids. Proceedings of the National Academy of Sciences U.S.A.: 81:3806–3810
     [Google Scholar]
  35. Zimber U., Adldinger H. K., Lenoir G. M., Vuillaume M., Von Knebel-Doeberitz M., Laux G., Desgranges C., Wittman P., Freese U. K., Schneider U., Bornkamm G. W. 1986; Geographical prevalence of two Epstein-Barr virus types. Virology 154:56–66
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-68-6-1587
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Nuclear DNA-binding Proteins Determined by the Epstein-Barr Virus-related Simian Lymphotropic Herpesviruses H. gorilla, H. pan, H. pongo and H. papio
J. Gen. Virol. 68, 1587 (1987); https://doi.org/10.1099/0022-1317-68-6-1587
/content/journal/jgv/10.1099/0022-1317-68-6-1587
/content/journal/jgv/10.1099/0022-1317-68-6-1587
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