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The double-stranded RNA genome of beet cryptic virus 1 (BCV 1) has been translated, after denaturation, in a wheat germ cell-free system. Two major polypeptides, of approximate M r 52000 and 67000, were produced. The smaller polypeptide, encoded by RNA 2 (M r 115 × 106), was immunoprecipitated by an antiserum against virus particles, and is therefore likely to be the coat protein; the larger polypeptide, encoded by RNA 1 (M r 1·36 × 106), is a likely candidate for the viral transcriptase. These two proteins represent most of the coding capacity of the BCV 1 genome (88 % for RN A 1 and 82 % for RNA 2), thus BCV 1 RNAs seem to act as monocistronic genes.
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