@article{mbs:/content/journal/jgv/10.1099/0022-1317-68-5-1417, author = "Accotto, Gian Paolo and Brisco, Michael J. and Hull, Roger", title = "In vitro Translation of the Double-stranded RNA Genome from Beet Cryptic Virus 1", journal= "Journal of General Virology", year = "1987", volume = "68", number = "5", pages = "1417-1422", doi = "https://doi.org/10.1099/0022-1317-68-5-1417", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-5-1417", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "dsRNA", keywords = "in vitro translation", keywords = "BCV", abstract = "Summary The double-stranded RNA genome of beet cryptic virus 1 (BCV 1) has been translated, after denaturation, in a wheat germ cell-free system. Two major polypeptides, of approximate M r 52000 and 67000, were produced. The smaller polypeptide, encoded by RNA 2 (M r 115 × 106), was immunoprecipitated by an antiserum against virus particles, and is therefore likely to be the coat protein; the larger polypeptide, encoded by RNA 1 (M r 1·36 × 106), is a likely candidate for the viral transcriptase. These two proteins represent most of the coding capacity of the BCV 1 genome (88 % for RN A 1 and 82 % for RNA 2), thus BCV 1 RNAs seem to act as monocistronic genes.", }