1887

Abstract

Summary

High multiplicity BK virus (BKV) infection of primary cells derived from human foetal pancreas resulted in massive cytopathology and subsequent outgrowth of cells. Intranuclear BKV T-antigen was present in all cells and viral antigen was detected in 10 to 30% of these cells. The subcultured cells yielded BKV in the supernatant (approx. 10 TCID/ml) and in the cells free viral DNA was present (approx. 10% of total cellular DNA content). Analysis of the viral DNA indicated the presence of deleted and rearranged BKV DNA molecules. Although all cells continuously expressed BKV T-antigen they did not exhibit the transformed phenotype. This persistent infection of human foetal pancreas cells represents a novel type of interaction between BKV and human cells which may correspond to the findings on BKV tropism for pancreatic cells.

Keyword(s): BK virus , pancreas and persistence
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/content/journal/jgv/10.1099/0022-1317-67-7-1485
1986-07-01
2022-01-25
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References

  1. Caputo A., Corallini A., Grossi M. P., Carrà L., Balboni P. G., Negrini M., Milanesi G., Federspil G., Barbanti-Brodano G. 1983; Episomal DNA of a BK virus variant in a human insulinoma. Journal of Medical Virology 12:37–49
    [Google Scholar]
  2. Coleman D. V. 1980; Recent developments in the papovaviruses: the human polyomaviruses (BK virus and JC virus). In Recent Advances in Clinical Virology pp 89–110 Edited by Waterson A. P. Edinburgh & New York: Churchill-Livingstone;
    [Google Scholar]
  3. Corallini A., Altavilla G., Cecchetti M. G., Fabris G., Grossi M. P., Balboni P. G., Lanza G., Barbanti-Brodano G. 1978; Ependymomas, malignant tumors of pancreatic islets, and osteosarcomas induced in hamsters by BK virus, a human papovavirus. Journal of the National Cancer Institute 61:875–880
    [Google Scholar]
  4. Corallini A., Altavilla G., Carrà L., Grossi M. P., Federspil G., Caputo A., Negrini M., Barbanti-Brodano G. 1982; Oncogenicity of BK virus for immunosuppressed hamsters. Archives of Virology 73:243–253
    [Google Scholar]
  5. Costa T., Yee C., Tralka T. S., Rabson A. S. 1976; Hamster ependymomas produced by intracerebral inoculation of a human papovavirus (MMV). Journal of the National Cancer Institute 56:863–864
    [Google Scholar]
  6. Fiori M., Dimayorca G. 1976; Occurrence of BK virus DNA in DNA obtained from certain human tumors. Proceedings of the National Academy of Sciences, U.S.A. 73:4662–4666
    [Google Scholar]
  7. Gardner S. D. 1973; Prevalence in England of antibody to human papovavirus (B.K.). British Medical Journal i:77–78
    [Google Scholar]
  8. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet i:1253–1257
    [Google Scholar]
  9. Grossi M. P., Meneguzzi G., Chenciner N., Corrallini A., Poli F., Altavilla G., Alberti S., Milanesi G., Barbanti-Brodano G. 1981; Lack of association between BK virus and ependymonas, malignant tumors of pancreatic islets, osteosarcomas and other human tumors. Intervirology 15:10–18
    [Google Scholar]
  10. Grossi M. P., Caputo A., Menguzzi G., Corallini A., Carrà L., Portolani M., Borgatti M., Milanesi G., Barbanti-Brodano G. 1982; Transformation of human embryonic fibroblasts by BK virus, BK virus DNA and a subgenomic BK virus DNA fragment. Journal of General Virology 63:393–403
    [Google Scholar]
  11. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  12. Huang A. S. 1973; Defective interfering viruses. Annual Review of Microbiology 27:101–117
    [Google Scholar]
  13. Israel M. A., Martin M. A., Takemoto K. K., Howley P. M., Aaronson S. A., Soloman D., Khoury G. 1978; Evaluation of normal and neoplastic human tissue for BK virus. Virology 90:187–196
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Noss G., Stauch G. 1984; Oncogenic activity of the BK type of human papova virus in inbred rat strains. Archives of Virology 81:41–51
    [Google Scholar]
  16. O’Neill F. S., Carroll D. 1983; Role of defective simian virus 40 genomes in establishment and maintenance of persistently infected primate cell lines. Intervirology 19:181–194
    [Google Scholar]
  17. Portolani M., Borgatti M., Corallini A., Cassai E., Grossi M. P., Barbanti-Brodano G., Possati L. 1978; Stable transformation of mouse, rabbit and monkey cells and abortive transformation of human cells by BK, a human papovavirus. Journal of General Virology 38:369–374
    [Google Scholar]
  18. Purchio A. F., Fareed G. C. 1979; Transformation of human embryonic kidney cells by human papovavirus BK. Journal of Virology 29:763–769
    [Google Scholar]
  19. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  20. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  21. Takemoto K. K., Linke H., Miyamura T., Fareed G. C. 1979; Persistent BK papovavirus infection of transformed human fetal brain cells. Journal of Virology 29:1177–1185
    [Google Scholar]
  22. Ter SCHEGGET J., Voves J., Van Strien A., Van der Noordaa J. 1980; Free viral DNA in BK virus-induced hamster tumor cells. Journal of Virology 35:331–339
    [Google Scholar]
  23. Uchida S., Watanabe S., Aizawa T., Furuno A., Muto T. 1979; Polyoncogenicity and insulinoma-inducing ability of BK virus, a human papovavirus, in Syrian golden hamsters. Journal of the National Cancer Institute 63:119–124
    [Google Scholar]
  24. Van der noordaa J. 1976; Infectivity, oncogenicity and transforming ability of BK virus and BK virus DNA. Journal of General Virology 30:371–373
    [Google Scholar]
  25. Van der noordaa J., Wertheim-Van Dillen P. 1977; Rise in antibodies to human papovavirus and clinical disease. British Medical Journal i:1471
    [Google Scholar]
  26. Watanabe S., Yoshiike K., Nozawa A., Yuasa U., Uchida S. 1979; Viable deletion mutant of human papovavirus BK that induces insulinomas in hamsters. Journal of Virology 32:934–942
    [Google Scholar]
  27. Wold W. S. M., Mackey J. K., Brachmann K. H., Takemori N., Riden P., Green M. 1978; Analysis of human tumors and human malignant cell lines for BK virus-specific DNA sequences. Proceedings of the National Academy of Sciences, U.S.A. 75:454–458
    [Google Scholar]
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