1887

Abstract

Summary

The nucleotide sequences of the spike precursor genes of infectious bronchitis virus strains M41 and 6/82 have been determined and compared with that of the Beaudette strain which we have previously sequenced. The two Massachusetts strains, M41 and Beaudette, were found to be remarkably similar, having only 3.7% of the amino acids different. The situation with 6/82, one of the new field isolates, is quite different and this strain had 13.8% of its amino acids different from Beaudette. The differences identified are discussed in terms of the structural features of the spike protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-12-2825
1986-12-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/12/JV0670122825.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-12-2825&mimeType=html&fmt=ahah

References

  1. Binns M. M., Boursnell M. E. G., Cavanagh D., Pappin D. J. C., Brown T. D. K. 1985a; Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV. Journal of General Virology 66:719–726
    [Google Scholar]
  2. Binns M. M., Boursnell M. E. G., Foulds I. J., Brown T. D. K. 1985b; The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus. Journal of Virological Methods 11:265–269
    [Google Scholar]
  3. Boursnell M. E. G., Binns M. M., Foulds I. J., Brown T. D. K. 1985; Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus. Journal of General Virology 66:573–580
    [Google Scholar]
  4. Brown T. D. K., Boursnell M. E. G., Binns M. M., Tomley F. M. 1986; Cloning and sequencing of 5′ terminal sequences from avian infectious bronchitis virus genomic RNA. Journal of General Virology 67:221–228
    [Google Scholar]
  5. Cook L. K. A. 1983; Isolation of a new serotype of infectious bronchitis-like virus from chickens in England. Veterinary Record 112:104–105
    [Google Scholar]
  6. Cook J. K. A. 1984; The classification of new serotypes of infectious bronchitis virus isolated from poultry flocks in Britain between 1981 and 1983. Avian Pathology 13:733–741
    [Google Scholar]
  7. Deininger P. L. 1983; Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Analytical Biochemistry 129:216–223
    [Google Scholar]
  8. Gething M. J., Bye J., Skehel J., Waterfield M. 1980; Cloning and DNA sequence of double-stranded copies of haemagglutinin genes from H2 and H3 strains elucidates antigenic shift and drift in human influenza virus. Nature, London 287:301–306
    [Google Scholar]
  9. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., Vandepol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  10. Minor P. D., Schild G. C., Bootman J., Evans D. M. A., Ferguson M., Reeve P., Spitz M., Stanway G., Cann A. J., Hauptmann R., Clarke L. D., Mountford R. C., Almond J. W. 1983; Location and primary structure of a major antigenic site for poliovirus neutralization. Nature, London 301:674–679
    [Google Scholar]
  11. Mockett A. P. A., Cavanagh D., Brown T. D. K. 1984; Monoclonal antibodies to the SI spike and membrane proteins of avian infectious bronchitis coronavirus strain Massachusetts M41. Journal of General Virology 65:2281–2286
    [Google Scholar]
  12. Niesters H. G. M., Zijderveld A. J., Seifert W. F., Lenstra J. A., Bleumink-Pluym N. M. C., Horzinek M. C., Van Der Zeist B. A. M. 1986; Infectious bronchitis virus RNA D encodes three potential translation products. Nucleic Acids Research 14:3144
    [Google Scholar]
  13. Porter A. G., Barber C., Carey N. H., Hallewell R. A., Threlfall G., Emtage J. S. 1979; Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA. Nature, London 282:471–477
    [Google Scholar]
  14. Van Regenmortel M. H. V. 1986; Which structural features determine protein antigenicity?. Trends in Biochemical Sciences 11:36–39
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-12-2825
Loading
/content/journal/jgv/10.1099/0022-1317-67-12-2825
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error