1887

Abstract

SUMMARY

A -specific antiserum was generated by injection of rabbits with a peptide predicted from the sequence of the v- gene of Moloney murine sarcoma virus (MuSV) strain 124. The peptide is composed of amino acids 37–55 (cyclized at the cysteine residues) conjugated to keyhole limpet haemocyanin. This serum [anti-(37–55c)] specifically recognized p37 in MuSV-124 acutely infected NIH-3T3 cells, P85 in 6m2 cells, an NRK clone infected with the temperature-sensitive mutant (110) of Moloney MuSV, and P100 in 54-5A4 cells, an NRK clone infected with a spontaneous revertant of 110. An additional protein of 55000 from uninfected cells was recognized by this serum. Reactivity of the serum toward the v--containing proteins and the 55K protein was completely inhibited by prior incubation with free peptide. The 55K protein was not recognized by antisera made from synthetic peptides prepared from the C-terminal eight or 12 amino acids of v-.

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1985-05-01
2022-01-26
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References

  1. Anderson S., Furth M., Wolff L., Ruscetti S. K., Sherr C. 1982; Monoclonal antibodies to the transformation-specific glycoprotein encoded by the feline retroviral oncogene v-fms.. Journal of Virology 44:696–702
    [Google Scholar]
  2. Arcement L. J., Karshin W. L., Naso R. B., Jamjoom G. A., Arlinghaus R. B. 1976; Biosynthesis of Rauscher leukemia viral proteins: presence of p30 and envelope p15 sequences in precursor polypeptides. Virology 69:763–774
    [Google Scholar]
  3. Arlinghaus R. B., Murphy E. C. Jr, Lyons D. D., Horn J. P., Mong S. M., Wood T. G. 1980; Translation products of the 124 strain of Moloney murine sarcoma virus (Mo-MuSV): characterization of a 23, 000 dalton candidate ‘src’ gene product. Animal Virus Genetics. ICN-UCLA Symposium on Molecular and Cellular Biology vol 18: pp 643–655 Edited by Fields B., Jaenisch R., F C. Fox, New York: Academic Press;
    [Google Scholar]
  4. Ball J. K., Huh T. Y., McCarter J. A. 1964; On the statistical distribution of epidermal papillomata in mice. British Journal of Cancer 18:120–123
    [Google Scholar]
  5. Ball J. K., McCarter J. A., Sunderland S. M. 1973; Evidence for helper independent murine sarcoma virus. I. Segregation of replication-defective viruses. Virology 56:268–284
    [Google Scholar]
  6. Bhatnagar P. K., Mao S. J. T., Gotto A. M. Jr, Sparrow J. T. 1983; The application of an improved solid phase synthetic technique to the delineation of an antigenic site of Apolipoprotein A-II. Peptides 4:343–349
    [Google Scholar]
  7. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  8. Brown M. D., Sen A., Sutcliffe J. G. 1984; Nucleotide sequence of the transforming gene of M1 murine sarcoma virus. Journal of Virology 49:579–582
    [Google Scholar]
  9. Dreesman G. R., Hollinger G. F., Sanchez Y., Oefinger P., Melnick J. L. 1981; Immunization of chimpanzees with hepatitis B virus-derived polypeptides. Infection and Immunity 32:61–67
    [Google Scholar]
  10. Edelstein M. S., McNair D. S., Sparrow J. T. 1981; The conversion of solid phase synthesizers to computer control. In PeptidesSynthesis, Structure, Function pp 217–220 Edited by Rich D. H., Gross E. Rockford: Pierce Chemical Company;
    [Google Scholar]
  11. Gallick G. E., Arlinghaus R. B. 1984; Incorporation of lipids into variants of Moloney sarcoma virus which produce gagmos fusion proteins. Virology 133:228–232
    [Google Scholar]
  12. Gattoni S., Kirschmeier P., Weinstein I. B., Escobedo J., Dina D. 1982; Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells. Molecular and Cellular Biology 2:42–51
    [Google Scholar]
  13. Hancock W. S., Capra J. D., Bradley W. A., Sparrow J. T. 1981; The use of reversed phase high performance liquid chromatography with radial compression for the analysis of peptide and protein mixtures. Journal of Chromatography 206:59–70
    [Google Scholar]
  14. Horn J. P., Wood T. G., Murphy E. C. Jr, Blair D. G., Arlinghaus R. B. 1981; A selective temperature-sensitive defect in viral RNA expression in cells infected with a ts transformation mutant of murine sarcoma virus. Cell 25:37–46
    [Google Scholar]
  15. Jamjoom G. A., Naso R. B., Arlinghaus R. B. 1977; Further characterization of intracellular precursor polyproteins of Rauscher leukemia virus. Virology 78:11–34
    [Google Scholar]
  16. Jones M., Bosselman R. A., Hoorn F. A. V. D., Berns A., Fan H., Verma I. M. 1980; Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells. Proceedings of the National Academy of Sciences, U. S. A 77:2651–2655
    [Google Scholar]
  17. Junghans R. P., Murphy E. C. Jr, Arlinghaus R. B. 1982; Electron microscopic analysis of ts110 Moloney mouse sarcoma virus, a variant of wild-type virus with two RNAs containing large deletions. Journal of Molecular Biology 161:229–255
    [Google Scholar]
  18. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  19. Kloetzer W. S., Maxwell S. A., Arlinghaus R. B. 1983; P85gag-mos encoded by tsl 10 Moloney murine sarcoma virus has an associated protein kinase activity. Proceedings of the National Academy of Sciences, U. S. A 80:412–416
    [Google Scholar]
  20. Kloetzer W. S., Maxwell S. A., Arlinghaus R. B. 1984; Further characterization of the P85gag-mos-associated protein kinase activity. Virology 138:143–155
    [Google Scholar]
  21. Muller R., Slamon D., Tremblay L., Cline M., Verma I. 1982; Differential expression of cellular oncogenes during pre and postnatal development of the mouse. Nature, London 299:640–644
    [Google Scholar]
  22. Murphy E. C. Jr, Arlinghaus R. B. 1982; Comparative tryptic peptide analysis of candidate P85gag-mos of ts110 Moloney murine sarcoma virus and P38-P23 mos gene-related proteins of wild-type virus. Virology 121:372–383
    [Google Scholar]
  23. Nadal-Ginard B. 1978; Commitment, fusion and biochemical differentiation of a myogenic cell line in the absence of DNA synthesis. Cell 15:855–864
    [Google Scholar]
  24. Nash M., Brown N. V., Wong J. L., Arlinghaus R. B., Murphy E. C. 1984; S1 nuclease mapping of viral RNAs from a temperature-sensitive transformation mutant of murine sarcoma virus. Journal of Virology 50:478–488
    [Google Scholar]
  25. Naso R. B., Arcement L. J., Arlinghaus R. B. 1975; Biosynthesis of Rauscher leukemia viral proteins. Cell 4:31–36
    [Google Scholar]
  26. Oskarsson M., McClements W. L., Blair D. G., Maizel J. V., Vande Woude G. F. 1980; Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus. Science 207:1222–1224
    [Google Scholar]
  27. Papkoff J., Hunter T. 1983; Detection of an 85, 000-dalton phosphoprotein in ts110 murine sarcoma virus-infected cells with antiserum against a v-mos peptide. Journal of Virology 45:1177–1182
    [Google Scholar]
  28. Papkoff J., Verma I. M., Hunter T. 1982; Detection of a transforming gene product of Moloney murine sarcoma virus in transformed cells. Cell 29:417–426
    [Google Scholar]
  29. Papkoff J., Nigg E. A., Hunter T. 1983; Transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein. Cell 33:161–172
    [Google Scholar]
  30. Rechavi T., Gluoe D., Canaani E. 1982; Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like element. Nature, London 300:607–611
    [Google Scholar]
  31. Sanchez Y., Ionescu I., Dreesman G. R., Kramp W., Six H., Hollinger F. B., Melnick J. 1980; Humoral and cellular immunity to hepatitis B-derived antigens: comparative activity of Freund complete adjuvant, alum, and liposomes. Infection and Immunity 30:728–733
    [Google Scholar]
  32. Sanchez Y., Ionescu L., Sparrow J., Melnick J., Dreesman G. 1982; Immunogenicity of conjugates and micelles of synthetic hepatitis B surface antigen peptides. Intervirology 18:209–213
    [Google Scholar]
  33. Sparrow J. T. 1976; An improved polystyrene support for solid phase peptide synthesis. Journal of Organic Chemistry 41:1350–1353
    [Google Scholar]
  34. Stanker L. H., Gallick G. E., Kloetzer W. S., Murphy E. C. Jr, Arlinghaus R. B. 1983a; A gag-mos polyprotein encoded by ts110 Moloney murine sarcoma virus. Journal of Virology 45:1183–1189
    [Google Scholar]
  35. Stanker L. H., Horn J. P., Gallick G. E., Kloetzer W. S., Murphy E. C. Jr, Blair D. G., Arlinghaus R. B. 1983b; gag-mos polyproteins encoded by variants of the Moloney strain of mouse sarcoma virus. Virology 126:336–347
    [Google Scholar]
  36. Stanker L. H., Gallick G. E., Horn J. P., Arlinghaus R. B. 1983c; P85gag-mos encoded by ts110 Moloney murine sarcoma virus: rapid turnover at the restrictive temperature. Journal of General Virology 64:2203–2211
    [Google Scholar]
  37. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proceedings of the National Academy of Sciences, U. S. A 76:4350–4354
    [Google Scholar]
  38. Van Beveren C. F., van Straaten J., Galleshaw A., Verma I. 1981; Nucleotide sequence of the genome of a murine sarcoma virus. Cell 27:97–108
    [Google Scholar]
  39. Wong P. K. Y., Gallick G. E. 1978; Preliminary characterization of a temperature-sensitive mutant of Moloney leukemia virus that produces particles at the restrictive temperature. Journal of Virology 25:187–192
    [Google Scholar]
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