%0 Journal Article %A Gallick, G. E. %A Sparrow, J. T. %A Singh, B. %A Maxwell, S. A. %A Stanker, L. H. %A Arlinghaus, R. B. %T Recognition of mos-related Proteins with an Antiserum to a Peptide of the v-mos Gene Product %D 1985 %J Journal of General Virology, %V 66 %N 5 %P 945-955 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-66-5-945 %K Mo-MuSV %K protein recognition %K v-mos gene %K peptide antibody %I Microbiology Society, %X SUMMARY A mos-specific antiserum was generated by injection of rabbits with a peptide predicted from the sequence of the v-mos gene of Moloney murine sarcoma virus (MuSV) strain 124. The peptide is composed of amino acids 37–55 (cyclized at the cysteine residues) conjugated to keyhole limpet haemocyanin. This serum [anti-mos(37–55c)] specifically recognized p37mos in MuSV-124 acutely infected NIH-3T3 cells, P85gag-mos in 6m2 cells, an NRK clone infected with the temperature-sensitive mutant (ts110) of Moloney MuSV, and P100gag-mos in 54-5A4 cells, an NRK clone infected with a spontaneous revertant of ts110. An additional protein of M r 55000 from uninfected cells was recognized by this serum. Reactivity of the serum toward the v-mos-containing proteins and the 55K protein was completely inhibited by prior incubation with free peptide. The 55K protein was not recognized by antisera made from synthetic peptides prepared from the C-terminal eight or 12 amino acids of v-mos. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-5-945