1887

Abstract

Summary

The mechanism of resistance of murine macrophages (Mø) to infection by herpes simplex virus type 1 (HSV-1) was examined. Infection of bone marrow-derived Mø (BMDMø) and resident peritoneal Mø (Res-Mø) was compared with infection of permissive Vero cells. In contrast to HSV-1 infection in Vero cells, no infectious virus was produced from either Mø cell type. However, marked cytopathic effect (c.p.e.) was evident in BMDMø at 48 h post-infection, while there was no c.p.e. at any time post-infection in the Res-Mø. Cloned RI subgenomic fragments representing the entire HSV-1 genome were used as probes in DNA:DNA hybridization experiments to determine the viral genome content in the infected cell types. In Res-Mø, HSV-1 DNA was present at early times post-infection but declined rapidly. In BMDMø, the virus genome was always detected and increased with time after infection. The results suggest that Res-Mø restrict HSV-1 production at a point prior to viral DNA synthesis, whereas the block in HSV production in BMDMø occurs at a later stage in the viral replicative cycle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-5-1123
1985-05-01
2019-10-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-5-1123
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error