1887

Abstract

SUMMARY

When type 1 poliovirions were extracted from infected HeLa cells and heated at 56 °C in the presence of 1 m-Mg, RNA-free, pluri-antigenic particles were formed. These particles possessed neutralization epitopes as well as epitopes associated with H antigen and with the isolated capsid protein VP3. The heating caused the formation of exclusively H antigenic particles when the Mg concentration was 10 or lower, or when the virus was further purified in order to remove remaining cellular components. The ability of purified virus to form pluri-antigenic particles upon heating could be restored by incubation at 37 °C with infected cell extract.

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/content/journal/jgv/10.1099/0022-1317-66-2-303
1985-02-01
2022-08-20
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