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Abstract
SUMMARY
When type 1 poliovirions were extracted from infected HeLa cells and heated at 56 °C in the presence of 1 mm-Mg2+, RNA-free, pluri-antigenic particles were formed. These particles possessed neutralization epitopes as well as epitopes associated with H antigen and with the isolated capsid protein VP3. The heating caused the formation of exclusively H antigenic particles when the Mg2+ concentration was 10−4 m or lower, or when the virus was further purified in order to remove remaining cellular components. The ability of purified virus to form pluri-antigenic particles upon heating could be restored by incubation at 37 °C with infected cell extract.
- Received:
- Accepted:
- Published Online:
© Journal of General Virology 1985