@article{mbs:/content/journal/jgv/10.1099/0022-1317-66-2-303, author = "Rombaut, Bart and Vrijsen, Raf and Boeyé, Albert", title = "Stabilization by Host Cell Components and Mg2+ of the Neutralization Epitopes of Poliovirus", journal= "Journal of General Virology", year = "1985", volume = "66", number = "2", pages = "303-307", doi = "https://doi.org/10.1099/0022-1317-66-2-303", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-66-2-303", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "heat inactivation", keywords = "pluri-antigenic particles", keywords = "poliovirus", abstract = "SUMMARY When type 1 poliovirions were extracted from infected HeLa cells and heated at 56 °C in the presence of 1 mm-Mg2+, RNA-free, pluri-antigenic particles were formed. These particles possessed neutralization epitopes as well as epitopes associated with H antigen and with the isolated capsid protein VP3. The heating caused the formation of exclusively H antigenic particles when the Mg2+ concentration was 10−4 m or lower, or when the virus was further purified in order to remove remaining cellular components. The ability of purified virus to form pluri-antigenic particles upon heating could be restored by incubation at 37 °C with infected cell extract.", }