1887

Abstract

SUMMARY

Na-K changes and cytotoxic effects were monitored in HeLa cells infected with encephalomyocarditis (EMC) virus, human rhinovirus or vesicular stomatitis virus (VSV). Whereas in all three cases Na-K changes followed viral inhibition of cellular protein synthesis, such changes preceded trypan blue staining of rhinovirus-infected or VSV-infected cells and paralleled trypan blue staining of EMC virus-infected cells. In each case a progressive reduction in cellular electrical volume was associated with Na-K changes. K leakage from EMC virus- or rhinovirus-infected cells clearly exceeded Na gain by these cells. These findings are basically similar to those previously reported for poliovirus-infected HeLa cells. It is concluded that Na-K changes induced by picornaviruses or VSV do not trigger shut-off of HeLa cell protein synthesis, but may contribute to late manifestations of cytotoxicity.

Keyword(s): HeLa cells , K changes , Na and shut-off
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-6-1135
1984-06-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/6/JV0650061135.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-6-1135&mimeType=html&fmt=ahah

References

  1. Carrasco L., Smith A. E. 1976; Sodium ions and the shutoff of host cell protein synthesis by picomaviruses. Nature, London 264:807–809
    [Google Scholar]
  2. Egberts E., Hackett P. B., Traub P. 1977; Alterations in the intracellular energetic and ionic conditions by mengovirus-infection of Ehrlich ascites tumor cells and its influence on protein synthesis in the midphase of infection. Journal of Virology 22:591–597
    [Google Scholar]
  3. Francoeur A. M., Stanners C. P. 1978; Evidence against the role of K+ in the shut-off of protein synthesis by vesicular stomatitis virus. Journal of General Virology 39:551–554
    [Google Scholar]
  4. Garry R. F., Waite M. R. F. 1979; Na+ and K + concentrations and the regulation of the interferon system in chick cells. Virology 96:121–128
    [Google Scholar]
  5. Garry R. F., Bishop J. M., Parker S., Westbrook K., Lewis G., Waite M. R. F. 1979; Na+ and K+ concentrations and the regulation of protein synthesis in Sindbis virus-infected chick cells. Virology 96:108–120
    [Google Scholar]
  6. Hackstadt T., Mallavia L. P. 1982; Sodium and potassium transport in herpes simplex virus-infected cells. Journal of General Virology 60:199–207
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  8. Nair C. N. 1981; Monovalent cation metabolism and cytopathic effects of poliovirus-infected HeLa cells. Journal of Virology 37:268–273
    [Google Scholar]
  9. Nair C. N., Stowers J. W., Singfield B. 1979; Guanidine sensitive Na+ accumulation in poliovirus-infected HeLa cells. Journal of Virology 31:184–189
    [Google Scholar]
  10. Norrie D. H., Wolstenholme J., Howcroft H., Stephen J. 1982; Vaccinia virus-induced changes in [Na+] and [K + ] in HeLa cells. Journal of General Virology 61:127–136
    [Google Scholar]
  11. Pasternak C. A., Micklem K. J. 1981; Virally induced alterations in cellular permeability: a basis of cellular physiological damage. Bioscience Reports 1:431–448
    [Google Scholar]
  12. Ramabhadran J. V., Thach R. E. 1981; Translational elongation rate changes in encephalomyocarditis virus-infected and interferon treated cells. Journal of Virology 39:573–583
    [Google Scholar]
  13. Schaefer A., Kuhne J., Zibirre R., Koch G. 1982; Poliovirus-induced alterations in HeLa cell membrane functions. Journal of Virology 44:444–449
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-65-6-1135
Loading
/content/journal/jgv/10.1099/0022-1317-65-6-1135
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error