%0 Journal Article %A Nair, C. N. %T Na and K Changes in Animal Virus-infected HeLa Cells %D 1984 %J Journal of General Virology, %V 65 %N 6 %P 1135-1138 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-65-6-1135 %K shut-off %K K changes %K Na %K HeLa cells %I Microbiology Society, %X SUMMARY Na-K changes and cytotoxic effects were monitored in HeLa cells infected with encephalomyocarditis (EMC) virus, human rhinovirus or vesicular stomatitis virus (VSV). Whereas in all three cases Na-K changes followed viral inhibition of cellular protein synthesis, such changes preceded trypan blue staining of rhinovirus-infected or VSV-infected cells and paralleled trypan blue staining of EMC virus-infected cells. In each case a progressive reduction in cellular electrical volume was associated with Na-K changes. K leakage from EMC virus- or rhinovirus-infected cells clearly exceeded Na gain by these cells. These findings are basically similar to those previously reported for poliovirus-infected HeLa cells. It is concluded that Na-K changes induced by picornaviruses or VSV do not trigger shut-off of HeLa cell protein synthesis, but may contribute to late manifestations of cytotoxicity. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-6-1135