1887

Abstract

SUMMARY

Condylomata acuminata and Buschke-Löwenstein tumours were analysed for the presence of human papillomavirus (HPV) transcripts. HPV DNA and RNA sequences were present in all 13 samples investigated. Ten contained HPV6 and three harboured HPV11. The HPV genomes were found exclusively as extrachromosomal circular molecules. In six biopsy specimens, viral RNA transcripts were not detectable by Northern blot analysis but could be demonstrated in dot blots. From seven HPV6-containing samples it was possible to obtain sufficient amounts of undegraded mRNA. We have found consistently one major species (1.4 kb). Less prominent species of 1.7, 1.85, 2.7 and 3.2 kb, respectively, were also detected. The 3′ ends of the HPV6 mRNAs were located between nucleotides 3917 and 4441 in the putative early region and between nucleotides 7232 and 7696 in the putative late region. The arrangement of the 3′ termini and the adjacent coding areas within the HPV6 genome show that the RNA species are transcribed from one DNA strand.

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1984-11-01
2024-04-15
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References

  1. Amtmann E., Sauer G. 1982; Bovine papillomavirus transcription: polyadenylated RNA species and assessment of the direction of transcription. Journal of Virology 43:59–66
    [Google Scholar]
  2. Boshart M., Gissmann L., Ikenberg H., Kleinheinz A., Scheurlen W., Zur Hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:1151–1157
    [Google Scholar]
  3. Denhardt D. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  4. De Villiers E.-M., Gissmann L., Zur Hausen H. 1981; Molecular cloning of viral DNA from human genital warts. Journal of Virology 40:932–935
    [Google Scholar]
  5. Engel L. W., Heilman C. A., Howley P. M. 1983; Transcriptional organization of bovine papillomavirus type 1. Journal of Virology 47:516–528
    [Google Scholar]
  6. Friedman E. Y., Rosbash M. 1977; The synthesis of high yields of full-length reverse transcripts of globin mRNA. Nucleic Acids Research 4:3455–3470
    [Google Scholar]
  7. Gissmann L., Zur Hausen H. 1980; Partial characterization of viral DNA from human genital warts (condylomata acuminata). International Journal of Cancer 25:605–609
    [Google Scholar]
  8. Gissmann L., De Villiers E.-M., Zur Hausen H. 1982a; Analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 DNA. International Journal of Cancer 29:143–146
    [Google Scholar]
  9. Gissmann L., Diehl V., Schultz-Coulon H.-L., Zur Hausen H. 1982b; Molecular cloning and characterization of human papillomavirus DNA derived from a laryngeal papilloma. Journal of Virology 44:393–400
    [Google Scholar]
  10. Gissmann L., Wolnik L., Ikenberg H., Koldovsky U., Schnurch H. G., Zur Hausen H. 1983; Human papil lomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proceedings of the National Academy of Sciences, U. S. A 80:560–563
    [Google Scholar]
  11. Grussendorf-Conen E. J., Gissmann L., Holters J. 1983; Correlation between content of viral DNA and evidence of mature virus particles in HPV-1, HPV-4, and HPV-6 induced virus acanthomata. Journal of Investigative Dermatology 81:511–513
    [Google Scholar]
  12. Ish-Horowicz D., Burke J. F. 1981; Rapid and efficient cosmid vector cloning. Nucleic Acids Research*2989–2998
    [Google Scholar]
  13. Krieg P., Amtmann E., Jonas D., Fischer H., Zang K., Sauer G. 1981; Episomal simian virus 40 genomes in human brain tumors. Proceedings of the National Academy of Sciences, U. S. A 78:6446–6450
    [Google Scholar]
  14. Krieg P., Amtmann E., Sauer G. 1983; The simultaneous extraction of high molecular weight DNA and of RNA from solid tumors. Analytical Biochemistry 134:288–294
    [Google Scholar]
  15. Pfister H. 1984; Biology and biochemistry of papillomaviruses. Reviews of Physiology, Biochemistry and Pharmacology 99:112–181
    [Google Scholar]
  16. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  17. Schwarz E., Durst M., Demankowski C., Lattermann O., Zech R., Wolfsperger E., Suhai S., Zur Hausen H. 1983; DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO Journal 2:2341–2348
    [Google Scholar]
  18. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Zur Hausen H. 1977; Human papillomaviruses and their possible role in squamous cell carcinomas. Current Topics in Microbiology and Immunology 78:1–30
    [Google Scholar]
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