1887

Abstract

SUMMARY

Virions of human cytomegalovirus were shown to contain two discrete membranes. An outer, loose fitting, membrane was sensitive to osmotic shock and could be partially removed by diluting buffered preparations of virions with water. Purified virions were shown, by SDS–polyacrylamide gel electrophoresis of virion membranes labelled by carbohydrate-specific procedures, to contain five glycoproteins with molecular weights of 52, 67, 95, 130 and 250, all × 10. Digestion of virions with endoglycosidases revealed that there were structural differences between the carbohydrate portions of the glycoproteins. All five glycoproteins were recognized by antibodies present in pools of human convalescent sera.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-11-1991
1984-11-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/11/JV0650111991.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-11-1991&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Clark B. R., Zaia J. A., Balce-Directo L., Ting Y. P. 1984; Isolation and partial chemical characterisation of a 64000 dalton glycoprotein of human cytomegalovirus. Journal of Virology 49:279–282
    [Google Scholar]
  3. Cohen G. L., Katz M., Hydrean-Stern C., Gisenberg R. J. 1978; Type common Gp-1 antigen of herpes simplex virus is associated with a 59000 molecular weight envelope glycoprotein. Journal of Virology 27:172–181
    [Google Scholar]
  4. Fiala M., Honess R. W., Heiner D. C., Heine J. W., Murnane J., Wallace R., Guze L. B. 1976; Cytomegalo-virus proteins. I. Polypeptides of virions and dense bodies. Journal of Virology 19:243–254
    [Google Scholar]
  5. Furukawa T., Gonczol E., Starr S., Tolpin M. D., Arbeter A., Plotkin S. A. 1984; HCMV envelope antigens induce both humoral and cellular immunity in guinea pig. Proceedings of the Society for Experimental Biology and Medicine 175:243–250
    [Google Scholar]
  6. Gahmberg C. G., Hakomori S. I. 1973; External labelling of cell surface galactose and galactosamine in glyco-lipid and glycoprotein of human erythrocytes. Journal of Biological Chemistry 248:4311–4317
    [Google Scholar]
  7. Gibson W. 1983; Protein counterparts of human and simian cytomegalovirus. Virology 128:391–406
    [Google Scholar]
  8. Gupta P., StJeor S., Rapp F. 1977; Comparison of the polypeptides of several strains of human cytomegalovirus. Journal of General Virology 34:447–454
    [Google Scholar]
  9. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194:495–496
    [Google Scholar]
  10. Irmiere A., Gibson W. 1983; Isolation and characterisation of a non-infectious virion-like particle released from cells infected with human strains of cytomegalovirus. Virology 130:118–133
    [Google Scholar]
  11. Kim K. S., Sapienza V. J., Carp R. I., Moon H. M. 1976; Analysis of structural polypeptides of purified human cytomegalovirus. Journal of Virology 20:604–611
    [Google Scholar]
  12. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Morgan A. J., Smith A. R., Barker R. N., Epstein M. A. 1984; A structural investigation of the Epstein-Barr (EB) virus membrane antigen glycoprotein, gp340. Journal of General Virology 65:397–404
    [Google Scholar]
  14. Norrild B. 1980; Immunochemistry of herpes simplex virus glycoproteins. Current Topics in Microbiology and Immunology 90:67–106
    [Google Scholar]
  15. North J. R., Morgan A. J., Thompson J. L., Epstein M. A. 1982; Purified EB virus gp 340 induces potent virus neutralising antibodies when incorporated in liposomes. Proceedings of the National Academy of Sciences, U. S. A 79:7504–7508
    [Google Scholar]
  16. Nowak B., Sullivan C., Sirnow P., Thomas R., Bircont F., Nicolas I. C., Fleckenstein B., Levine A. J. 1984; Characterisation of monoclonal antibodies and polyclonal immune sera directed against human cytomegalovirus virion proteins. Virology 132:325–338
    [Google Scholar]
  17. Pereira L., Hoffman M., Cremer N. 1982; Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus infected cell extracts by human sera. Infection and Immunity 36:933–942
    [Google Scholar]
  18. Plummer G. 1973; Cytomegaloviruses of man and animals. Progress in Medical Virology 15:92–125
    [Google Scholar]
  19. Reynolds D. W., Stagno S., Alford C. A. 1979; Laboratory diagnosis of cytomegalovirus infections. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections 5th edn pp 399–439 Edited by Lennette E. H., Schmidt N. J. Washington, D.C.: American Public Health Association;
    [Google Scholar]
  20. Severi B., Landini M. P., Musiahi M., Zerbini M. 1979; A study of the passage of human cytomegalovirus from the nucleus to the cytoplasm. Microbiologica 2:265–273
    [Google Scholar]
  21. Smith J. D., De Harven E. 1973; Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. I.Sequence of viral replication. Journal of Virology 12:919–930
    [Google Scholar]
  22. Spear P. G. 1980; Cell membranes and viral envelopes. In Herpesviruses vol 2: pp 709–750 Edited by Blough H. A., Tiffany J. M. New York: Academic Press;
    [Google Scholar]
  23. Stinski M. F. 1976; Human cytomegalovirus: glycoproteins associated with virions and dense bodies. Journal of Virology 19:594–609
    [Google Scholar]
  24. Stinski M. F. 1977; Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus. Journal of Virology 23:751–767
    [Google Scholar]
  25. Stinski M. F. 1978; Sequence of protein synthesis in cells infected by human cytomegalovirus. Early and late virus-induced polypeptides. Journal of Virology 26:686–701
    [Google Scholar]
  26. Stinski M. F., Mocarski E. S., Thomsen D. R., Urbanowski M. L. 1979; Membrane glycoproteins and antigens induced by human cytomegalovirus. Journal of General Virology 43:119–129
    [Google Scholar]
  27. Strange R. E., Powell E. O., Pearce T. W. 1971; The rapid detection and determination of sparse bacterial populations with radioactively labelled homologous antibodies. Journal of General Microbiology 67:349–357
    [Google Scholar]
  28. Talbot P., Almeida J. D. 1977; Human cytomegalovirus: purification of enveloped virions and dense bodies. Journal of General Virology 36:345–349
    [Google Scholar]
  29. Thorley-Lawson D. A., Geilinger K. 1980; Monoclonal antibodies against the major glycoprotein (gp 350/220) of Epstein-Barr virus neutralise infectivity. Proceedings of the National Academy of Sciences, U. S. A 77:5307–5311
    [Google Scholar]
  30. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U. S. A 76:4350–4354
    [Google Scholar]
  31. Weller T. H. 1971; The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. New England Journal of Medicine 285:203–214
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-11-1991
Loading
/content/journal/jgv/10.1099/0022-1317-65-11-1991
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error