1887

Abstract

SUMMARY

Mesophyll protoplasts were prepared from plants and infected with cowpea mosaic virus (CPMV) or cymbidium ringspot virus (CyRSV) using inocula containing either polyethylene glycol or poly--ornithine. The level of infection was estimated by fluorescent antibody staining and by spot hybridization assay. About 25% of the protoplasts became infected with CPMV and about 35% with CyRSV. Yields of progeny RNA were about 700 ng of CPMV RNA and 3 µg of CyRSV RNA per 10 protoplasts. Cytopathic structures found in protoplasts inoculated with CPMV or CyRSV were similar to the structures found in CPMV-infected cowpea protoplasts or in CyRSV-infected plants respectively.

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/content/journal/jgv/10.1099/0022-1317-65-10-1851
1984-10-01
2022-01-23
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