@article{mbs:/content/journal/jgv/10.1099/0022-1317-65-10-1851, author = "de Varennes, Amarilis and Russo, Marcello and Maule, Andrew J.", title = "Infection of Protoplasts from Chenopodium Quinoa with Cowpea Mosaic and Cymbidium Ringspot Viruses", journal= "Journal of General Virology", year = "1984", volume = "65", number = "10", pages = "1851-1855", doi = "https://doi.org/10.1099/0022-1317-65-10-1851", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-10-1851", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "cowpea mosaic virus", keywords = "Chenopodium quinoa", keywords = "cymbidium ringspot virus", keywords = "protoplasts", abstract = "SUMMARY Mesophyll protoplasts were prepared from Chenopodium quinoa plants and infected with cowpea mosaic virus (CPMV) or cymbidium ringspot virus (CyRSV) using inocula containing either polyethylene glycol or poly-l-ornithine. The level of infection was estimated by fluorescent antibody staining and by spot hybridization assay. About 25% of the protoplasts became infected with CPMV and about 35% with CyRSV. Yields of progeny RNA were about 700 ng of CPMV RNA and 3 µg of CyRSV RNA per 106 protoplasts. Cytopathic structures found in C. quinoa protoplasts inoculated with CPMV or CyRSV were similar to the structures found in CPMV-infected cowpea protoplasts or in CyRSV-infected C. quinoa plants respectively.", }