1887

Abstract

SUMMARY

A peptide, prepared by gentle oxidative detoxification of a methionine-free cobra α-neurotoxin, was evaluated for antiviral activities and . When added to BHK cells before infection the peptide reduced herpes simplex virus type 1 (HSV-1)-induced TCD in a dose- and time-dependent manner. The antiviral effect was also expressed on initiation of cell treatment 1 h after infection if the peptide was left in contact with cells. Single, intracerebral doses of the toxoid in suckling mice significantly increased 50% survival times, and reduced virus replication in the infected brain tissues. Cutaneous lesions and resultant scar formation induced by HSV-1 in hairless mice were also significantly reduced by subcutaneous peptide injections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-7-1475
1983-07-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/7/JV0640071475.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-7-1475&mimeType=html&fmt=ahah

References

  1. Bonner J., Chalkley G. R., Dahmers M., Fambrough D., Fujimura F., Huang R. C., Huberman J., Jensen R., Marushige K., Ohlenbusch H., Olivera B., Widhold J. 1968; Isolation and characterization of chromosomal nucleoproteins. Methods in Enzymology 12:32–37
    [Google Scholar]
  2. Chatman V. B., Dimari S. J. 1974; Use of sulphopropyl-Sephadex chromatography and disc electrophoresis for the fractionation and identification of Elapid venoms. Toxicon 12:405–414
    [Google Scholar]
  3. Gabriel O. 1972; Analytical disc electrophoresis. Methods in Enzymology 42:565–578
    [Google Scholar]
  4. Karlsson E., Arnberg H., Eaker D. 1971; Isolation of the principal neurotoxins of two Naja naja subspecies. European Journal of Biochemistry 21:1–16
    [Google Scholar]
  5. Klein R. J., Friedman-Kien A. E., Brady E. 1974; Treatment of poxvirus infections in rabbits with 9-/1-D-arabinofuranosyladenine. Antimicrobial Agents and Chemotherapy 5:409–412
    [Google Scholar]
  6. Lentz T. L., Burrage T. G., Smith A. L., Crick J., Tignor G. H. 1982; Is the acetylcholine receptor a rabies virus receptor?. Science 215:182–184
    [Google Scholar]
  7. Miller K. D., Miller G. G., Sanders M., Fellowes O. N. 1977; Inhibition of virus-induced plaque formation by atoxic derivatives of purified cobra neurotoxins. Biochimica et biophysica acta 496:192–196
    [Google Scholar]
  8. Moore S., Stein W. H. 1954; A modified ninhydin reagent for the photometric determination of amino acids and related compounds. Journal of Biological Chemistry 211:907–913
    [Google Scholar]
  9. Reed L. J., Muench H. 1938; A simple method for estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  10. Sanders M., Soret M. G., Akin B. A. A., Roizin L. 1958; Neurotoxoid interference in Macacus rhesus infected intramuscularly with poliovirus. Science 127:594–596
    [Google Scholar]
  11. Tu A. T. 1973; Neurotoxins of animal venoms: snakes. Annual Review of Biochemistry 42:235–258
    [Google Scholar]
  12. Walkinshaw M. D., Saenger W., Maelicke A. 1980; Three-dimensional structure of the ‘long’ neurotoxin from cobra venom. Proceedings of the National Academy of Sciences, U. S. A 77:2400–2404
    [Google Scholar]
  13. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulfate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  14. Yourist J., Miller K., Haines H. 1979; Inhibition of herpes simplex virus replication by cobra a-neurotoxoid. Abstracts, seventh meeting of the International Society for Neurochemistry (Jerusalem Israel p 999
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-7-1475
Loading
/content/journal/jgv/10.1099/0022-1317-64-7-1475
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error