Skip to content
1887

Abstract

SUMMARY

A peptide, prepared by gentle oxidative detoxification of a methionine-free cobra α-neurotoxin, was evaluated for antiviral activities and . When added to BHK cells before infection the peptide reduced herpes simplex virus type 1 (HSV-1)-induced TCD in a dose- and time-dependent manner. The antiviral effect was also expressed on initiation of cell treatment 1 h after infection if the peptide was left in contact with cells. Single, intracerebral doses of the toxoid in suckling mice significantly increased 50% survival times, and reduced virus replication in the infected brain tissues. Cutaneous lesions and resultant scar formation induced by HSV-1 in hairless mice were also significantly reduced by subcutaneous peptide injections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-7-1475
1983-07-01
2025-11-19

Metrics

Loading full text...

Full text loading...

References

  1. Bonner J., Chalkley G. R., Dahmers M., Fambrough D., Fujimura F., Huang R. C., Huberman J., Jensen R., Marushige K., Ohlenbusch H., Olivera B., Widhold J. 1968; Isolation and characterization of chromosomal nucleoproteins. Methods in Enzymology 12:32–37
    [Google Scholar]
  2. Chatman V. B., Dimari S. J. 1974; Use of sulphopropyl-Sephadex chromatography and disc electrophoresis for the fractionation and identification of Elapid venoms. Toxicon 12:405–414
    [Google Scholar]
  3. Gabriel O. 1972; Analytical disc electrophoresis. Methods in Enzymology 42:565–578
    [Google Scholar]
  4. Karlsson E., Arnberg H., Eaker D. 1971; Isolation of the principal neurotoxins of two Naja naja subspecies. European Journal of Biochemistry 21:1–16
    [Google Scholar]
  5. Klein R. J., Friedman-Kien A. E., Brady E. 1974; Treatment of poxvirus infections in rabbits with 9-/1-D-arabinofuranosyladenine. Antimicrobial Agents and Chemotherapy 5:409–412
    [Google Scholar]
  6. Lentz T. L., Burrage T. G., Smith A. L., Crick J., Tignor G. H. 1982; Is the acetylcholine receptor a rabies virus receptor?. Science 215:182–184
    [Google Scholar]
  7. Miller K. D., Miller G. G., Sanders M., Fellowes O. N. 1977; Inhibition of virus-induced plaque formation by atoxic derivatives of purified cobra neurotoxins. Biochimica et biophysica acta 496:192–196
    [Google Scholar]
  8. Moore S., Stein W. H. 1954; A modified ninhydin reagent for the photometric determination of amino acids and related compounds. Journal of Biological Chemistry 211:907–913
    [Google Scholar]
  9. Reed L. J., Muench H. 1938; A simple method for estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  10. Sanders M., Soret M. G., Akin B. A. A., Roizin L. 1958; Neurotoxoid interference in Macacus rhesus infected intramuscularly with poliovirus. Science 127:594–596
    [Google Scholar]
  11. Tu A. T. 1973; Neurotoxins of animal venoms: snakes. Annual Review of Biochemistry 42:235–258
    [Google Scholar]
  12. Walkinshaw M. D., Saenger W., Maelicke A. 1980; Three-dimensional structure of the ‘long’ neurotoxin from cobra venom. Proceedings of the National Academy of Sciences, U. S. A 77:2400–2404
    [Google Scholar]
  13. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulfate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  14. Yourist J., Miller K., Haines H. 1979; Inhibition of herpes simplex virus replication by cobra a-neurotoxoid. Abstracts, seventh meeting of the International Society for Neurochemistry (Jerusalem Israel p 999
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-64-7-1475
Loading
/content/journal/jgv/10.1099/0022-1317-64-7-1475
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error