Treatment of Daudi cells with successively increasing concentrations of interferon-α resulted in the selection of a cell population which multiplied in the continued presence of 10 units/ml of interferon-α. A number of clones of interferon-resistant Daudi cells were isolated from this population. Two clones, DIF and DIF, were found to exhibit moderate and pronounced resistance, respectively, to both the antiviral and antiproliferative actions of human interferons-α and -β. These clones were also less responsive to the enhancement by interferon of Epstein—Barr virus early antigen expression. Both the surface antigens and karyotype of the interferon-resistant clones were similar to those of parental Daudi cells. After prolonged cultivation in the absence of interferon, DIF cells were found to ‘revert’ to an intermediate interferon sensitivity. The interferon sensitivity of clone DIF remained unchanged even after more than 1 year in culture.


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