1887

Abstract

SUMMARY

Human α-interferons (IFN-αs) made in bacteria were examined for antiviral activity against herpes simplex virus type 2 (HSV-2) infections of mouse L-cells , and acute cervicovaginal and lethal systemic HSV-2 infections of BALB/c mice. The recombinant DNA-derived hybrid interferon IFN-αAD(Bgl) showed pronounced antiviral activity , exceeding the activity of either of the parental subtypes IFN-αA and IFN-αD and that of the other hybrids IFN-αAD(Pvu). A combination of topical and systemic treatments with IFN-αA and IFN-αAD(Bgl) failed to protect mice from subsequent challenge with an acute cervicovaginal infection of HSV-2. Protection from lethal systemic HSV-2 infection in mice was observed when IFN-αAD(Bgl) and IFN-αAD(Pvu) were administered systemically, whereas IFN-αA failed to confer protection. These results suggest that for protection against infection with HSV-2, the routes of introduction of the virus and of the interferon influence the host response to interferon therapy.

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1983-10-01
2022-07-01
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References

  1. Dandoy F., Demaeyer-guignard J., Bailey D., Demaeyer E. 1982; Mouse genes influence antiviral action of interferon in vivo. Infection and Immunity 38:89–93
    [Google Scholar]
  2. Fish E. N., Tobin S. M., Wilson W. D., Papsin F. R. 1979; Ascending infection following cervicovaginal exposure to herpesvirus hominis type II. Obstetrics and Gynecology 53:429–436
    [Google Scholar]
  3. Fish E. N., Tobin S. M., Cooter N. B. E., Papsin F. R. 1982; Update on the relation of herpesvirus hominis type II to carcinoma of the cervix. Obstetrics and Gynecology 59:220–224
    [Google Scholar]
  4. Fish E. N., Banerjee K., Stebbing N. 1983; Human leukocyte interferon subtypes have different anti-proliferative activities on human cells. Biochemical and Biophysical Research Communications 112:537–546
    [Google Scholar]
  5. Goeddel D. V., Yelverton E., Ullrich A., Heyneker H. L., Miozzari G., Holmes H., Seeburg P. H., Dull T., May L., Stebbing N., Crea R., Maeda S., McCandliss R., Sloma A., Tabor J. M., Gross M., Familletti P. C., Pestka S. 1980; Human leukocyte interferon produced by E. coli is biologically active. Nature, London 287:411–416
    [Google Scholar]
  6. Goeddel D. V., Leung D. W., Dull T. I., Gross M., Lawn R. M., McCandliss R., Seeburg P. H., Ullrich A., Yelverton E., Gray P. W. 1981; The structure of eight distinct cloned human leukocyte interferon cDNAs. Nature, London 290:20–26
    [Google Scholar]
  7. Grabner G., Smolin G., OLumoto M., Stebbing N. 1983; Antiviral effects of highly purified bacterial-derived human leukocyte interferons (subtypes A and D) and a human fibroblast interferon against herpesvirus infection of the rabbit eye. Archives of Ophthalmology (in press)
    [Google Scholar]
  8. Hannigan G. E., Gewert D. R., Fish E. N., Read S. E., Williams B. R. G. 1983; Differential binding of human interferon-α subtypes to receptors on lymphoblastoid cells. Biochemical and Biophysical Research Communications 110:537–544
    [Google Scholar]
  9. Kapoor A. K., Nash A. A., Wildy P., Phelan J., Mclean C. S., Field H. J. 1982; Pathogenesis of herpes simplex virus in congenitally athymic mice: the relative roles of cell-mediated and humoral immunity. Journal of General Virology 60:225–233
    [Google Scholar]
  10. Lee S. H., Kelley S., Chiu H., Stebbing N. 1982a; Stimulation of natural killer cell activity and inhibition of proliferation of various leukemic cells by purified human leukocyte interferon subtypes. Cancer Research 42:1312–1314
    [Google Scholar]
  11. Lee S. H., Weck P. K., Moore J., Chen S., Stebbing N. 1982b; Pharmacological comparison of two hybrid recombinant DNA-derived human leukocyte interferons. In Chemistry and Biology of Interferons, UCLA Symposia on Molecular Cellular Biology vol 25: pp 341–351 Edited by Merigan T., Friedman R., Fox C. F. New York: Academic Press;
    [Google Scholar]
  12. Nagata S., Taira H., Hall A., Johnsund L., Streuli M., Escodi J., Boll W., Cantell K., Weissmann C. 1980; Synthesis in E. coli of a polypeptide with human leukocyte interferon activity. Nature, London 284:316–320
    [Google Scholar]
  13. Smolin G., Stebbing N., Friedlaender M., Friedlaender R., Okumoto M. 1982; Natural and cloned human leukocyte interferon in herpesvirus infections of rabbit eyes. Archives of Ophthalmology 100:481–483
    [Google Scholar]
  14. Streuli M., Hall A., Boll W., Stewart W. E., Nagata S., Weissmann C. 1981; Target Cell Specificity of two species of human interferon-a produced in Escherichia coli and of hybrid molecules derived from them. Proceedings of the National Academy of Sciences, U. S. A 78:2848–2852
    [Google Scholar]
  15. Tobin S. M., Wilson W. D., Fish E. N., Papsin F. R. 1978; Relation of herpesvirus hominis type II to carcinoma of the cervix. An animal model for the induction of long-term latency of herpesvirus hominis type II. Obstetrics and Gynecology 51:707–712
    [Google Scholar]
  16. Weck P. K., Apperson S., May L., Stebbing N. 1981a; Comparison of the antiviral activity of various cloned human interferon-α subtypes in mammalian cell cultures. Journal of General Virology 57:233–237
    [Google Scholar]
  17. Weck P. K., Apperson S., Stebbing N., Gray P. W., Leung D., Shepard H. M., Goeddel D. V. 1981b; Antiviral activities of hybrids of two major leukocyte interferons. Nucleic Acids Research 9:6153–6166
    [Google Scholar]
  18. Weck P. K., Rinderknecht E., Estell D. A., Stebbing N. 1982; Antiviral activity of bacteria-derived human leukocyte interferons against encephalomyocarditis virus infection of mice. Infection and Immunity 35:660–665
    [Google Scholar]
  19. Yelverton E., Leung D., Weck P. K., Gray P. W., Goeddel D. V. 1981; Bacterial synthesis of a novel human leukocyte interferon. Nucleic Acids Research 9:731–741
    [Google Scholar]
  20. Zawatzky R., Gresser I., Demaeyer E., Kirchner H. 1982; The role of interferon in the resistance of C57BL/6 mice to various doses of herpes simplex virus type 1. Journal of Infectious Diseases 146:405–410
    [Google Scholar]
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