RT Journal Article SR Electronic(1) A1 Fish, E. N. A1 Banerjee, K. A1 Stebbing, N.YR 1983 T1 Antiviral Activities of Cloned Human Leukocyte Interferons against Herpes Simplex Virus Type 2 Infections of Mice JF Journal of General Virology, VO 64 IS 10 SP 2317 OP 2321 DO https://doi.org/10.1099/0022-1317-64-10-2317 PB Microbiology Society, SN 1465-2099, AB SUMMARY Human α-interferons (IFN-αs) made in bacteria were examined for antiviral activity against herpes simplex virus type 2 (HSV-2) infections of mouse L-cells in vitro, and acute cervicovaginal and lethal systemic HSV-2 infections of BALB/c mice. The recombinant DNA-derived hybrid interferon IFN-αAD(Bgl) showed pronounced antiviral activity in vitro, exceeding the activity of either of the parental subtypes IFN-αA and IFN-αD and that of the other hybrids IFN-αAD(Pvu). A combination of topical and systemic treatments with IFN-αA and IFN-αAD(Bgl) failed to protect mice from subsequent challenge with an acute cervicovaginal infection of HSV-2. Protection from lethal systemic HSV-2 infection in mice was observed when IFN-αAD(Bgl) and IFN-αAD(Pvu) were administered systemically, whereas IFN-αA failed to confer protection. These results suggest that for protection against infection with HSV-2, the routes of introduction of the virus and of the interferon influence the host response to interferon therapy., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-10-2317