1887

Abstract

SUMMARY

The RNA components of chronic bee-paralysis virus (CPV) and those of chronic bee-paralysis virus associate (CPVA) have been compared. CPV has five singlestranded RNA components, two larger RNAs designated 1 (mol. wt. 1.35 × 10; 4200 nucleotides) and 2 (mol. wt. 0.9 × 10; 2800 nucleotides), and three smaller RNAs designated 3a, 3b and 3c, each with mol. wt. 0.35 × 10 (1100 nucleotides). CPVA has three single-stranded RNA components designated A, B and C, each with mol. wt. 0.35 × 10 (1100 nucleotides). Gel electrophoretic and T fingerprint analyses have shown that RNAs 3a, 3b and 3c are very similar to, and probably identical to, RNAs A, B and C respectively, i.e. these three RNAs appear to be encapsidated in both CPV and CPVA particles. A positive correlation was observed between the proportion of RNAs 3a, 3b and 3c in CPV particles and the amount of CPVA (and hence RNAs A, B and C) which had replicated. Fingerprint analysis has shown that RNAs A, B and C are distinct but related and also that about 50% of the sequence of each these three RNAs is homologous with RNA 2. It is therefore possible that, although CPV and CPVA are serologically unrelated, the RNAs of CPVA have evolved from CPV RNA 2.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-1-171
1982-11-01
2022-08-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/1/JV0630010171.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-1-171&mimeType=html&fmt=ahah

References

  1. Bailey L. 1976; Viruses attacking the honeybee. Advances in Virus Research 20:271–304
    [Google Scholar]
  2. Bailey L., Woods R. D. 1974; Three previously undescribed viruses from the honey bee. Journal of General Virology 25:175–186
    [Google Scholar]
  3. Bailey L., Woods R. D. 1977; Two more small RNA viruses from honey bees and further observations on sacbrood and acute bee-paralysis viruses. Journal of General Virology 37:175–182
    [Google Scholar]
  4. Bailey L., Gibbs A. I., Woods R. D. 1963; Two viruses from adult honey bees (Apis mellifera Linnaeus). Virology 21:390–395
    [Google Scholar]
  5. Bailey L., Gibbs A. J., Woods R. D. 1968; The purification and properties of chronic bee-paralysis virus. Journal of General Virology 2:251–260
    [Google Scholar]
  6. Bailey L., Ball B. V., Carpenter J. M., Woods R. D. 1980; Small virus-like particles in honey bees associated with chronic paralysis virus and with a previously undescribed disease. Journal of General Virology 46:149–155
    [Google Scholar]
  7. Buck K. W., Ratti G. 1977; Molecular weight of double-stranded RNA: a re-examination of Aspergillus foetidus virus S RNA components. Journal of General Virology 37:215–219
    [Google Scholar]
  8. Dodds A. J., Hamilton W. I. 1976; Structure interactions between viruses as a consequence of mixed infections. Advances in Virus Research 20:33–86
    [Google Scholar]
  9. Francki R. I. B., Mossop D. W., Hatta T. 1979; Cucumber mosaic virus. Commonwealth Mycological Institute/ Association of Applied Biologists Descriptions of Plant Viruses no. 213
    [Google Scholar]
  10. Hilleman B., Morris T. J. 1981; Comparison of chronic bee paralysis virus to several multicomponent plant viruses. Phytopathology 71:880–881
    [Google Scholar]
  11. Kennedy S. I. T. 1976; Sequence relationships between the genome and the intracellular RNA species of standard and defective-interfering Semliki Forest virus. Journal of Molecular Biology 108:491–511
    [Google Scholar]
  12. Loening U. E. 1968; Molecular weights of ribosomal RNA in relation to evolution. Journal of Molecular Biology 38:355–365
    [Google Scholar]
  13. Mcmaster G. K., Carmichael G. G. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences of the United Stales of America 74:4835–4838
    [Google Scholar]
  14. Mossop D. W., Francki R. I. B. 1978; Survival of a satellite RNA in vivo and its dependence on cucumber mosaic virus for its replication. Virology 86:562–566
    [Google Scholar]
  15. Murant A. F., Taylor M., Duncan G. H., Raschke J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after dénaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  16. Peterson J.F., Brakke M. K. 1973; Genomic masking in mixed infections with brome mosaic and barley stripe mosaic viruses. Virology 51:174–182
    [Google Scholar]
  17. Reijnders L., Sloof P., Sival J., Borst P. 1973; Gel electrophoresis of RNA under denaturing conditions. Biochimica et biophysica acta 324:320–333
    [Google Scholar]
  18. Schuerch A. R., Mitchell W. R., Joklik W. K. 1975; Isolation of intact individual species of single- and doublestranded RNA after fractionation by polyacrylamide gel electrophoresis. Analytical Biochemistry 65:331–345
    [Google Scholar]
  19. Szekely M., Sanger F. 1969; Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids. Journal of Molecular Biology 43:607–617
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-1-171
Loading
/content/journal/jgv/10.1099/0022-1317-63-1-171
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error