1887

Abstract

SUMMARY

Six cell fusion-causing mutants were isolated from the (101 to syn-106) and three from the (syn-107 to syn-109) strains of herpes simplex virus type 1 (-1). The mutants were studied by complementation and recombination with syn-20 (a syncytial mutant of ) and -B5 (a syncytial mutant of ). Some studies also employed MP, a syncytium-inducing strain isolated from the non-syncytial parent, mP. Complementation and recombination of -20 and B5 indicated that these two mutants were altered in two different virus genes. The recombination frequency between -20 and -B5 was very similar to that observed between MP and -B5, indicating that -20 and may represent alterations in the same virus gene, -101, -103, -104 and -105 were tentatively assigned to the -20 complementation group, while -107 and -109 were tentatively assigned to the fs-B5 complementation group, -106 and -108 were excluded from the -B5 group, -102 could not be excluded from either complementation group, -101 induced markedly less fusion at 38 °C relative to 34 °C. At 34 °C the patterns of -101-infected cell peptides and glycopeptides, examined by -gel electrophoresis, were normal, but at 38 °C the amount of glycopeptide gC was particularly reduced, -102 produced decreased amounts of glycoproteins, and a non-glycosylated peptide, probably ICP6, was absent from extracts infected with -106.

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/content/journal/jgv/10.1099/0022-1317-61-2-245
1982-08-01
2022-01-22
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