%0 Journal Article %A Bond, Vincent C. %A Person, Stanley %A Warner, Susan C. %T The Isolation and Characterization of Mutants of Herpes Simplex Virus Type 1 that Induce Cell Fusion %D 1982 %J Journal of General Virology, %V 61 %N 2 %P 245-254 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-61-2-245 %K cell fusion %K complementation %K HSV-1 %K recombination %K syn mutants %I Microbiology Society, %X SUMMARY Six cell fusion-causing syn mutants were isolated from the KOS (syn-101 to syn-106) and three from the HFEM (syn-107 to syn-109) strains of herpes simplex virus type 1 (HSV-1). The mutants were studied by complementation and recombination with syn-20 (a syncytial mutant of KOS) and ts-B5 (a syncytial mutant of HFEM). Some studies also employed MP, a syncytium-inducing strain isolated from the non-syncytial parent, mP. Complementation and recombination of syn-20 and ts-B5 indicated that these two mutants were altered in two different virus genes. The recombination frequency between syn-20 and ts-B5 was very similar to that observed between MP and ts-B5, indicating that syn-20 and MP may represent alterations in the same virus gene, syn-101, syn-103, syn-104 and syn-105 were tentatively assigned to the syn-20 complementation group, while syn-107 and syn-109 were tentatively assigned to the fs-B5 complementation group, syn-106 and syn-108 were excluded from the ts-B5 group, syn-102 could not be excluded from either complementation group, syn-101 induced markedly less fusion at 38 °C relative to 34 °C. At 34 °C the patterns of syn-101-infected cell peptides and glycopeptides, examined by SDS-gel electrophoresis, were normal, but at 38 °C the amount of glycopeptide gC was particularly reduced, syn-102 produced decreased amounts of glycoproteins, and a non-glycosylated peptide, probably ICP6, was absent from extracts infected with syn-106. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-61-2-245