1887

Abstract

SUMMARY

Replication of hepatitis A virus (HAV) in the human hepatoma-derived PLC/PRF/5 cell line was neither inhibited in the presence of various concentrations of guanidine or D-2-(α-hydroxybenzyl)benzimidazole (D-HBB), nor were the two chemicals effective in combination. Under identical conditions, however, replication of poliovirus type 1 was inhibited. Tracer experiments with radiolabelled guanidine and D-HBB also furnished no evidence that the two antiviral substances were metabolized gradually to inactive derivatives in PLC/PRF/5 cells. Therefore, it is concluded that resistance to the action of guanidine and D-HBB is an inherent characteristic of HAV. However, the insensitivity of HAV to these drugs does not exclude the virus from the family of picornaviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-61-1-111
1982-07-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/61/1/JV0610010111.html?itemId=/content/journal/jgv/10.1099/0022-1317-61-1-111&mimeType=html&fmt=ahah

References

  1. Alexander J, Macnab G., Saunders R. 1978; Studies of in vitro production of hepatitis B surface antigen by a human hepatoma cell line. Perspectives in Virology 10:103–120
    [Google Scholar]
  2. Caliguiri L. A., Tamm I. 1973 Guanidine and 2-(α-hydroxybenzyl) benzimidazole (HBB): selective inhibitors of picornavirus multiplication. In Selective Inhibitors of Viral Function pp. 257–293 Edited by Carter W. A. Florida: CRC Press;
    [Google Scholar]
  3. Coulepis A. G., Locarnini S. A., Gust I. D. 1980; Iodination of hepatitis A virus reveals a fourth structural polypeptide. Journal of Virology 35:572–574
    [Google Scholar]
  4. Coulepis A. G., Tannock G. A., Locarnini S. A., Gust I. D. 1981; Evidence that the genome of hepatitis A virus consists of single-stranded RNA. Journal of Virology 37:473–477
    [Google Scholar]
  5. Eggers H. J. 1982 Selective inhibition of virus replication by benzimidazoles in cell culture and in the organism. In Handbook of Experimental Pharmacology Wien: Springer-Verlag; (in press)
    [Google Scholar]
  6. Eggers H. J., Tamm I. 1961 a; Spectrum and characteristics of the virus inhibitory action of 2-(α-hydroxybenzyl)-benzimidazole. Journal of Experimental Medicine 113:657–682
    [Google Scholar]
  7. Eggers H. J., Tamm I. 1961 b; 2-(a-hydroxybenzyl)-benzimidazole (HBB) as an aid in virus classification. Virology 13:545–546
    [Google Scholar]
  8. Eggers H. J., Tamm I. 1962; On the mechanism of selective inhibition of enterovirus multiplication by 2-(α-hydroxybenzyl)-benzimidazole. Virology 26:426–438
    [Google Scholar]
  9. Flehmig B. 1980; Hepatitis A virus in cell culture. I. Propagation of different hepatitis A virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4). Medical Microbiology and Immunology 168:239–248
    [Google Scholar]
  10. FrÖsner G. G., Deinhardt F., Scheid R., Gauss-mÜller V., Holmes N., Messelberger V., Siegl G., Alexander J. J. 1979; Propagation of human hepatitis A virus in a hepatoma cell line. Infection 7:303–306
    [Google Scholar]
  11. Provost P. J., Hilleman M. R. 1979; Propagation of human hepatitis A virus in cell culture in vitro. Proceedings of the Society for Experimental Biology and Medicine 160:213–221
    [Google Scholar]
  12. Sergiescu D., Horodniceanu F., Aubert-Combiescu A. 1972; The use of inhibitors to study picornavirus genetics. Progress in Medical Virology 14:123–199
    [Google Scholar]
  13. Siegl G., FrÖsner G. G. 1978; Characterization and classification of virus particles associated with hepatitis A. II. Type and configuration of nucleic acid. Journal of Virology 26:48–53
    [Google Scholar]
  14. Siegl G., FrÖsner G. G., Gauss-MÜller V., Tratschin J. D., Deinhardt F. 1981; The physicochemical properties of infectious hepatitis A virions. Journal of General Virology 57:331–341
    [Google Scholar]
  15. Tamm I., Eggers H. J. 1963; Specific inhibition of replication of animal viruses. Science 142:24–33
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-61-1-111
Loading
/content/journal/jgv/10.1099/0022-1317-61-1-111
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error