1887

Abstract

SUMMARY

Although the Pr65 precursor polyproteins of Moloney murine leukaemia virus (M-MuLV) and of Rauscher murine leukaemia virus (R-MuLV) have the same apparent mol. wt. by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), their initial ∼40000 dalton intermediate cleavage products differ in mol. wt., i.e. the M-MuLV product (Pr41.5) is 1500 daltons larger than the R-MuLV product (Pr40). We took advantage of this difference to show that cleavage of R-MuLV Pr65 by the M-MuLV proteolytic activity gives rise to R-MuLV Pr40 and not M-MuLV Pr41.5. This result suggests that the specificity for cleavage of the MuLV Pr65 is built into the substrate.

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/content/journal/jgv/10.1099/0022-1317-54-1-33
1981-05-01
2022-09-25
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