1887

Abstract

SUMMARY

Although the Pr65 precursor polyproteins of Moloney murine leukaemia virus (M-MuLV) and of Rauscher murine leukaemia virus (R-MuLV) have the same apparent mol. wt. by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), their initial ∼40000 dalton intermediate cleavage products differ in mol. wt., i.e. the M-MuLV product (Pr41.5) is 1500 daltons larger than the R-MuLV product (Pr40). We took advantage of this difference to show that cleavage of R-MuLV Pr65 by the M-MuLV proteolytic activity gives rise to R-MuLV Pr40 and not M-MuLV Pr41.5. This result suggests that the specificity for cleavage of the MuLV Pr65 is built into the substrate.

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1981-05-01
2024-04-26
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References

  1. Bolognesi D. P., Luftig R. B., Shaper J. 1973; Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures. Virology 56:549–564
    [Google Scholar]
  2. Jamjoom G. A., Naso R. B., Arlinghaus R. B. 1976; Selective decrease in the rate of cleavage of an intracellular precursor to Rauscher leukemia virus p30 by treatment of infected cells with actinomycin D. Journal of Virology 19:1054–1072
    [Google Scholar]
  3. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  4. Naso R. B., Karshin W. L., Yu Y. H., Arlinghaus R. B. 1979; Characterization of 40000 and 25000 dalton intermediate precursors to Rauscher murine leukemia virus gag gene products. Journal of Virology 32:187–198
    [Google Scholar]
  5. Ng V. L., Kopchick J. J., Karshin W. L., Wood T. F., Arlinghaus R. B. 1980; The structural relatedness of the virus core proteins of Rauscher and Moloney murine leukaemia viruses. Journal of General Virology 47:161–170
    [Google Scholar]
  6. Oroszlan S., Gilden R. 1980; Primary structure analysis of retrovirus proteins. In Molecular Biology of RNA Tumor Viruses pp 299–344 Edited by Stephenson J. S. New York: Academic Press;
    [Google Scholar]
  7. Yoshinaka Y., Luftig R. B. 1977a; Partial characterization of a P70 proteolytic factor that is present in purified virions of Rauscher leukemia virus (RLV). Biochemical and Biophysical Research Communications 76:54–63
    [Google Scholar]
  8. Yoshinaka Y., Luftig R. B. 1977b; Characterization of Rauscher leukemia virus (RLV) P40-42, an intermediate cleavage product of the group specific antigen (gag) precursor polypeptide, P65-70. Biochemical and Biophysical Research Communications 79:319–325
    [Google Scholar]
  9. Yoshinaka Y., Luftig R. B. 1977c; Properties of a P70 proteolytic factor of murine leukemia viruses. Cell 12:709–719
    [Google Scholar]
  10. Yoshinaka Y., Luftig R. B. 1980; Physicochemical characterization and specificity of the murine leukaemia virus Pr65gag proteolytic factor. Journal of General Virology 48:329–340
    [Google Scholar]
  11. Yoshinaka Y., Ishigame K., Ohno T., Kageyama S., Shibata K., Luftig R. B. 1980; Preparations enriched for ‘immature’ murine leukemia virus particles that remain in tissue culture fluids are deficient in Pr65gag proteolytic activity. Virology 100:130–140
    [Google Scholar]
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