@article{mbs:/content/journal/jgv/10.1099/0022-1317-54-1-33, author = "Yoshinaka, Y. and Luftig, R. B.", title = "Cleavage of Rauscher Leukaemia Virus (R-MuLV) Pr65gag by the Moloney Leukaemia Virus (M-MuLV) Proteolytic Activity Produces the R-MuLV-specific but not the M-MuLV-specific 40000 Dalton Intermediate Polypeptide", journal= "Journal of General Virology", year = "1981", volume = "54", number = "1", pages = "33-38", doi = "https://doi.org/10.1099/0022-1317-54-1-33", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-54-1-33", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Although the Pr65gag precursor polyproteins of Moloney murine leukaemia virus (M-MuLV) and of Rauscher murine leukaemia virus (R-MuLV) have the same apparent mol. wt. by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), their initial ∼40000 dalton intermediate cleavage products differ in mol. wt., i.e. the M-MuLV product (Pr41.5gag) is 1500 daltons larger than the R-MuLV product (Pr40gag). We took advantage of this difference to show that in vitro cleavage of R-MuLV Pr65gag by the M-MuLV proteolytic activity gives rise to R-MuLV Pr40gag and not M-MuLV Pr41.5gag. This result suggests that the specificity for cleavage of the MuLV Pr65gag is built into the substrate.", }