1887

Abstract

SUMMARY

Five polypeptides were found when purified influenza virus was analysed by electrophoresis on polyacrylamide gel. Previous work has shown that hyperimmune convalescent antibody prepared in mice and adsorbed with purified influenza virus which had been disrupted with ether still gave precipitin lines in immunodiffusion tests with extracts of cells infected with influenza virus. These presumed non-structural antigens, labelled with radioactive amino acids, were precipitated with the virus-adsorbed antibody preparation. A comparison of the precipitate and purified virus by polyacrylamide gel electrophoresis revealed three polypeptides which differed from the polypeptides of the virion. These were probably virus specific non-structural proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-4-499
1969-12-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/4/JV0050040499.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-4-499&mimeType=html&fmt=ahah

References

  1. Ada G. L., Perry B. T. 1954; The nucleic acid content of influenza virus. Aust. J. exp. Biol. med. Sci 32:453
    [Google Scholar]
  2. Barry R. D., Ives D. R., Cruickshank J. G. 1962; Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature, Lond 194:1139
    [Google Scholar]
  3. Cebra J. J., Givol D., Katchalski E. 1962; Soluble complexes of antigen and antibody fragments. J. biol. Chem 237:751
    [Google Scholar]
  4. Davis B. J. 1964); Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci 121:404
    [Google Scholar]
  5. Dimmock N. J. 1969; New virus-specific antigens in cells infected with influenza virus. Virology (in the Press)
    [Google Scholar]
  6. Duesberg P. H., Robinson H. L., Robinson W. S., Huebner R. J., Turner H. C. 1968; Proteins of Rous sarcoma virus. Virology 36:73
    [Google Scholar]
  7. Griffith I. P. 1968; The isolation and characterisation of the structural proteins of influenza virus. Proc. Aust. Biochem. Soc p. 37
    [Google Scholar]
  8. Hoyle L. V. 1968 The influenza viruses. Virology Monographs no 4, Eds.. Gard S., Hallauer C., Meyer K. F. Springer-Verlag:
    [Google Scholar]
  9. Joss A., Hay A. J., Skehel D. C., Burke D. C. 1968; Protein components of fowl plague virus. Biochem. J 110:40P
    [Google Scholar]
  10. Laver W. G. 1962; The structure of influenza viruses. I. N-terminal amino acid analyses. Virology 18:19
    [Google Scholar]
  11. Laver W. G., Webster R. G. 1966; The structure of influenza viruses. IV. Chemical studies of the host antigen. Virology 30:104
    [Google Scholar]
  12. Lowry D. H., Roseborough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin-Phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  13. Ornstein L. 1964; Disc electrophoresis. I. Background and theory. Ann. N.Y. Acad. Sci 121:321
    [Google Scholar]
  14. Pons M. W., Hirst G. K. 1968; Polyacrylamide gel electrophoresis of influenza virus RNA. Virology 34:386
    [Google Scholar]
  15. Scharff M. D., Shatkin A. J., Levintow L. 1963; Association of newly formed viral protein with specific polyribosomes. Proc. natn. Acad. Sci. U.S.A. 50:686
    [Google Scholar]
  16. Shapiro A. E., Vinuela E., Maizel J. V. 1967; Molecular weights of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem. biophys. Res. Commun 28:815
    [Google Scholar]
  17. Summers D. F., Maizel J. V. 1968; Evidence for large precursor proteins in poliovirus synthesis. Proc. natn. Acad. Sci. U.S.A. 59:966
    [Google Scholar]
  18. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific non capsid proteins in poliovirus-infected HeLa cells. Proc. natn. Acad. Sci. U.S.A. 54:505
    [Google Scholar]
  19. Watson D. H. 1969; The separation of herpes virus-specific antigens by polyacrylamide gel electrophoresis. J. gen. Virol 4:151
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-5-4-499
Loading
/content/journal/jgv/10.1099/0022-1317-5-4-499
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error