The nature of the refractoriness of C6 glioblastoma cells to herpes simplex virus type 1 (HSV-1) infection has been studied. The cells were restricted in susceptibility to HSV-1 since only a small proportion of the cells could be infected by HSV-1 and the virus yield per cell was low. The susceptibility to infection was increased by treating the cells with trypsin-EDTA prior to infection. The cells so treated recovered resistance to the virus when incubated at 37 °C, their resistance being restored to the initial level in 2 days. This restoration was inhibited by addition of cycloheximide or puromycin. Trypsin-EDTA treatment of C6 cells increased the efficiency of adsorption of HSV-1 and the formation of stable cell-virus complexes from which the virus could not be dissociated by heparin.


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