%0 Journal Article %A Ando, Tamie %A Arai, Hiroshi %T Stimulation of Herpes Simplex Type 1 Infection of C6 Cells by Trypsin-EDTA %D 1980 %J Journal of General Virology, %V 48 %N 2 %P 319-328 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-48-2-319 %I Microbiology Society, %X SUMMARY The nature of the refractoriness of C6 glioblastoma cells to herpes simplex virus type 1 (HSV-1) infection has been studied. The cells were restricted in susceptibility to HSV-1 since only a small proportion of the cells could be infected by HSV-1 and the virus yield per cell was low. The susceptibility to infection was increased by treating the cells with trypsin-EDTA prior to infection. The cells so treated recovered resistance to the virus when incubated at 37 °C, their resistance being restored to the initial level in 2 days. This restoration was inhibited by addition of cycloheximide or puromycin. Trypsin-EDTA treatment of C6 cells increased the efficiency of adsorption of HSV-1 and the formation of stable cell-virus complexes from which the virus could not be dissociated by heparin. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-48-2-319