1887

Abstract

SUMMARY

Gel filtration chromatography of disrupted bovine leukaemia virus (BLV) resulted in the isolation of the 25000 mol. wt. major internal protein (p25), two previously uncharacterized proteins of mol. wt. 65000 (p65) and 12000 (p12), and a mixture of p12 and a protein of mol. wt. 15000 (p15). The p65 protein does not bind to concanavalin A and its antigenicity is ether resistant. Therefore, this polypeptide is different from the previously described glycoprotein associated with BLV. Radioimmunoprecipitation and competitive radioimmunoassays indicated that the p65 protein shares antigenic determinants with the p25, p15 and p12 proteins, respectively. Furthermore, tryptic peptide mapping demonstrated that p65 contains p25, p15, p12 and a BLV protein of mol. wt. 10000 (p10). These results are consistent with the view that p65 is the precursor of gag gene-derived core proteins of BLV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-47-2-311
1980-04-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/47/2/JV0470020311.html?itemId=/content/journal/jgv/10.1099/0022-1317-47-2-311&mimeType=html&fmt=ahah

References

  1. Aarcement L. J., Karshin W. L., Naso R. B., Arlinghaus R. B. 1977; ‘gag’ Polyprotein precursor of Rauscher murine leukemia virus. Virology 81:284–297
    [Google Scholar]
  2. Baliga V., Ferrer J. F. 1977; Expression of the bovine leukemia virus and its internal antigen in blood lymphocytes. Proceedings of the Society for Experimental Biology and Medicine 156:388–391
    [Google Scholar]
  3. Barbacid M., Stephenson J. R., Aaronson S. A. 1976a; Structural polypeptides of mammalian type-C RNA viruses. Journal of Biological Chemistry 251:4859–4866
    [Google Scholar]
  4. Barbacid M., Stephenson J. R., Aaronson S. A. 1976b; gag Gene of mammalian type-C RNA tumour viruses. Nature, London 262:554–559
    [Google Scholar]
  5. Bech-Welson S., Piper C. E., Ferrer J. F. 1978; Natural mode of transmission of the bovine leukemia virus: role of bloodsucking insects. American Journal of Veterinary Research 39:1089–1092
    [Google Scholar]
  6. Calafat J., Hageman P. C., Ressang A. A. 1974; Structure of C-type virus particles in lymphocyte cultures of bovine origin. Journal of the National Cancer Institute 52:1251–1257
    [Google Scholar]
  7. Callahan R., Lieber M. M., Todaro G. J., Graves D. C., Ferrer J. F. 1976; Bovine leukemia virus gene in the DNA of leukemic cattle. Science 192:1005–1006
    [Google Scholar]
  8. Dev Are S. G., Stephenson J. R. 1977; Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. Journal of Virology 23:443–447
    [Google Scholar]
  9. Devare S. G., Stephenson S. R., Sarma P. S., Aaronson S. A. 1976; Bovine lymphosarcoma: development of a radioimmunologic technique for detection of the etiologic agent. Science 194:1428–1430
    [Google Scholar]
  10. Diglio C. A., Ferrer J. R. 1976; Induction of syncytia by the bovine C-type leukemia virus. Cancer Research 36:1056–1067
    [Google Scholar]
  11. Duesberg P. H., Robinson L. H., Robinson W. S. 1968; Proteins of Rous sarcoma virus. Virology 36:73–86
    [Google Scholar]
  12. Elder J. H., Pickett R. A., Hampton J., Lerner R. A. 1977; Radioiodination of proteins in single polyacrylamide gel slices. Journal of Biological Chemistry 252:6510–6515
    [Google Scholar]
  13. Ferrer J. F. 1972; Antigenic comparison of bovine type-C virus with murine and feline leukemia viruses. Cancer Research 32:1871–1877
    [Google Scholar]
  14. Ferrer J. F., Diglio C. A. 1976; Development of an in vitro infectivity assay for the C. type bovine leukemia virus. Cancer Research 36:1068–1073
    [Google Scholar]
  15. Ferrer J. F., Stock N. D., Lin P. S. 1971; Detection of replicating C-type viruses in continuous cell cultures established from cows with leukemia: effect of the culture medium. Journal of the National Cancer Institute 27:613–621
    [Google Scholar]
  16. Ferrer J. F., Avila L., Stock N. D. 1972; Serological detection of type-C viruses found in bovine cultures. Cancer Research 32:1864–1870
    [Google Scholar]
  17. Ferrer J. F., Abt D. A., Bhatt D. M., Marshak R. R. 1974; Studies on the relationship between infection with bovine C-type virus, leukemia and persistent lymphocytosis in cattle. Cancer Research 321:893–900
    [Google Scholar]
  18. Ferrer J. F., Baliga V., Diglio C., Graves D., Kenyon S. J., McDonald H., Piper C., Wuu K. 1976a; Recent studies on the characterization of the bovine leukemia virus (BLV): development of new methods for the diagnosis of BLV infection. Veterinary Microbiology 1:159–184
    [Google Scholar]
  19. Ferrer J. F., Piper C. E., Abt D. A., Marshak R. R., Bhatt D. M. 1976b; Natural transmission of the bovine C type leukemia virus (BLV). Bibliotheca Haematologica 43:235–237
    [Google Scholar]
  20. Ferrer J. F., Piper C. E., Abt D. A., Marshak R. R. 1977; Diagnosis of bovine leukemia virus infection: evaluation of serologic and hematologic tests by a direct infectivity detection assay. American Journal of Veterinary Research 38:1977–1981
    [Google Scholar]
  21. Frenzel B., Kaaden O. R., Mussgay M. 1977; Purification of a glycoprotein from bovine leukemia virus (BLV). Zeitschrift für Naturforschung 32C:301–304
    [Google Scholar]
  22. Ghysdael J., Kettmann R., Burny A. 1979; Translation of bovine leukemia virus virion RNAs in heterologous protein-synthesizing systems. Journal of Virology 29:1087–1098
    [Google Scholar]
  23. Graves D. C., Diglio C. A., Ferrer J. F. 1977; A reverse transcriptase assay for detection of the bovine leukemia virus. American Journal of Veterinary Research 38:1739–1744
    [Google Scholar]
  24. Graves D. C., Ferrer J. F. 1976; In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. Cancer Research 36:4152–4159
    [Google Scholar]
  25. Hunter W. M. 1967 In Handbook of Experimental Immunology pp 608–642 Edited by Weir D. M.
    [Google Scholar]
  26. Jamjoom G., Karshin W. L., Naso R. B., Arcement L. J., Arlinghaus R. B. 1975; Proteins of Rauscher murine leukemia virus: resolution of a 70000 dalton, non-glycosylated polypeptide containing p30 peptide sequences. Virology 68:135–145
    [Google Scholar]
  27. Kaaden O. R., Frenzel B., Dietzschold B., Weiland F., Mussgay M. 1977; Isolation of a p15 polypeptide from bovine leukemia virus and detection of specific antibodies in leukemic cattle. Virology 77:501–509
    [Google Scholar]
  28. McDonald H. C., Ferrer J. F. 1976; Detection, quantitation and characterization of the major internal antigen of the bovine leukemia virus by radioimmunoassay. Journal of the National Cancer Institute 57:875–882
    [Google Scholar]
  29. Miller J. M., van Der Maaten M. J. 1976; Sociologic detection of bovine leukemia virus infection. Veterinary Microbiology 1:195–202
    [Google Scholar]
  30. Mirault M. E., Scherrer K. 1971; Isolation of preribosomes from HeLa cells and their characterization by electrophoresis on uniform and exponential-gradient-polyacrylamide gels. European Journal of Biochemistry 23:372–386
    [Google Scholar]
  31. Okasinski G. F., Velicer L. F. 1977; Analysis of intracellular feline leukemia virus proteins. II. Generation of feline virus structural proteins from precursor polypeptides. Journal of Virology 22:74–85
    [Google Scholar]
  32. Onuma M., Olson C., Driscoll D. M. 1976; Properties of two isolated antigens associated with bovine leukemia virus infection. Journal of the National Cancer Institute 57:571–578
    [Google Scholar]
  33. Oskarsson M. K., Robey W. G., Harris C. L., Fischinger P. J., Haapala D. K., van De Woude G. F. 1975; A p6o polypeptide in the feline leukemia virus pseudotype of Moloney sarcoma virus with murine leukemia virus P30 antigenic determinants. Proceedings of the National Academy of Sciences of the United States of America 72:2380–2384
    [Google Scholar]
  34. Phillips M., Miller J. M., Van Der Maaten M. J. 1978; Isolation of a precipitating glycoprotein antigen from cell cultures persistently infected with bovine leukemia virus. Journal of the National Cancer Institute 60:213–217
    [Google Scholar]
  35. Piper C. E., Abt D. A., Ferrer J. F., Marshak R. R. 1975; Seroepidemiological evidence for horizontal transmission of bovine C-type virus. Cancer Research 35:2714–2716
    [Google Scholar]
  36. Ponce De Leon M., Hessel H., Cohen G. H. 1973; Separation of Herpes simplex virus induced antigens by concanavalin A affinity chromatography. Journal of Virology 12:744–766
    [Google Scholar]
  37. Pritchett R., Zee Y. C. 1975; Structural proteins of bovine viral diarrhea virus. American Journal of Veterinary Research 36:1731–1737
    [Google Scholar]
  38. Stock N. D., Ferrer J. F. 1972; Replicating C-type virus in phytohemagglutinin-treated buffy coat cultures of bovine origin. Journal of the National Cancer Institute 48:985–996
    [Google Scholar]
  39. Strand M., August J. T. 1973; Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. Journal of Virology 13:171–180
    [Google Scholar]
  40. Vogt V. M., Eisenman R. 1973; Identification of a large polypeptide precursor of avian oncornavirus proteins. Proceedings of the National Academy of Sciences of the United States of America 70:1734–1738
    [Google Scholar]
  41. Vogt V. M., Eisenman R., Diggelmann H. 1975; Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide. Journal of Molecular Biology 96:471–493
    [Google Scholar]
  42. Von Der Helm K. 1977; Cleavage of Rous sarcoma viral polypeptide precursor into internal structural proteins in vitro involves viral protein p15. Proceedings of the National Academy of Sciences of the United States of America 74:911–915
    [Google Scholar]
  43. Wuu K. D., Graves D. C., Ferrer J. F. 1977; Inhibition of the reverse transcriptase of bovine leukemia virus by antibody in sera from leukemic cattle and immunological characterization of the enzyme. Cancer Research 37:1438–1442
    [Google Scholar]
  44. Yoshinaka Y., Luftig R. B. 1977a; Properties of a P70 proteolytic factor of murine leukemia viruses. Cell 12:709–719
    [Google Scholar]
  45. Yoshinaka Y., Luftig R. B. 1977b; Murine leukemia virus morphogenesis: cleavage of p70 in vitro can be accompanied by a shift from a concentrically coiled internal strand (immature) to a collapsed (mature) form of the virus core. Proceedings of the National Academy of Sciences of the United States of America 74:3446–3450
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-47-2-311
Loading
/content/journal/jgv/10.1099/0022-1317-47-2-311
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error