1887

Abstract

SUMMARY

Semliki Forest, Sindbis and rubella viruses can fuse erythrocytes from several different species. Large fusion vesicles consisting of tens to hundreds of red blood cells were seen under optimal conditions. For the haemagglutination and cell fusion to occur the adsorption of virus and further incubation had to be carried out at pH 5.8. Haemagglutination took place over a wide temperature range (0 to 40 °C) whereas fusion required temperatures between 37 and 42 °C. Haemolysis of red blood cells induced by togaviruses also required initial incubation at pH 5.8 to enable attachment of the virus to occur after which the pH of the buffer could be raised to neutrality without inhibiting the haemolysis. The amount of togaviruses and Sendai virus required to fuse red blood cells was about the same [1 haemagglutinating unit (HAU)/ml] but different ionic conditions were required for fusion.

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1980-02-01
2022-01-20
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References

  1. Apostolov K., Almeida J. D. 1972; Interaction of Sendai (HVJ) virus with human erythrocytes: a morphological study of haemolysis cell fusion. Journal of General Virology 15:227–234
    [Google Scholar]
  2. Bachi T., Aguet M., Howe C. 1973; Fusion of erythrocytes by Sendai virus studied by immuno-freeze-etching. Journal of Virology n:1004–1012
    [Google Scholar]
  3. CLarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  4. Dalrymple J. M., Schlesinger S., Russell P. K. 1976; Antigenic characterization of two Sindbis envelope glycoproteins separated by isoelectric focusing. Virology 69:93–103
    [Google Scholar]
  5. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167–182
    [Google Scholar]
  6. Fries E., Helenius A. 1979; Binding of Semliki Forest virus and its spike glycoproteins to cells. European Journal of Biochemistry 97:213–220
    [Google Scholar]
  7. Fukai K., Suzuki T. 1955; On the characteristics of a newly isolated hemagglutinating virus from mice. Medical Journal of Osaka University 6:1–15
    [Google Scholar]
  8. Hall W. W., Martin S. J. 1974; The biochemical and biological characteristics of the surface components of measles virus. Journal of General Virology 22:363–374
    [Google Scholar]
  9. Halonen P. E., Ryan J. M., Stewart J. A. 1967; Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK-21 cells. Proceedings of the Society for Experimental Biology and Medicine 125:162–167
    [Google Scholar]
  10. Helenius A., Von Bonsdorff C.-H. 1976; Semliki Forest virus membrane proteins: preparation and characterization of spike complexes soluble in detergent free medium. Biochimica et Biophysica Acta 436:895–899
    [Google Scholar]
  11. Helenius A., Fries E., Garoff H., Simons K. 1976; Solubilization of the Semliki Forest virus membrane with sodium deoxycholate. Biochimica et Biophysica Acta 436:319–334
    [Google Scholar]
  12. Helenius A., Morein B., Fries E., Simons K., Robinson P., Schirrmacher V., Terhorst C., Strominger J. L. 1978; Human (HLA-A and HLA-B) and murine (H-2K and H-2D) histocompatibility antigens are cell surface receptors for Semliki Forest virus. Proceedings of the National Academy of Sciences of the United States of America 75:3846–3850
    [Google Scholar]
  13. Homma M., Shimizu K., Shimizu Y., Ishida N. 1976; On the study of Sendai virus hemolysis. I. Complete Sendai virus lacking in hemolytic activity. Virology 71:41–47
    [Google Scholar]
  14. Hosaka Y., Shimizu Y. K. 1972; Artificial assembly of envelope particles of HVJ. I. Assembly of hemolytic and fusion factors from envelopes solubilized by Nonidet P 40. Virology 49:627–639
    [Google Scholar]
  15. Karabatsos N. 1963; Hemolytic properties of eastern and western equine encephalitis viruses. Journal of Immunology 91:76–82
    [Google Scholar]
  16. Karabatsos N. 1965; Further studies on the hemolytic properties of arboviruses. Proceedings of the Society for Experimental Biology and Medicine 118:461–465
    [Google Scholar]
  17. Karabatsos N. 1973; Density gradient characterization of disrupted and untreated eastern encephalitis virus. Archiv fur die Gesamte Virusforschung 40:222–235
    [Google Scholar]
  18. Knutton S. 1977; Studies of membrane fusion. II. Fusion of human erythrocytes by Sendai virus. Journal of Cell Science 28:189–210
    [Google Scholar]
  19. Knutton S., Jackson D., Ford M. 1977; Studies of membrane fusion. I. Paramyxovirus-induced cell fusion, a scanning electron-microscope study. Journal of Cell Science 28:179–188
    [Google Scholar]
  20. Kobayashi N. 1978; Hemolytic activity of rubella virus. Virology 89:610–612
    [Google Scholar]
  21. Poste G., Allison A. C. 1973; Membrane fusion. Biochimica et Biophysica Acta 300:421–465
    [Google Scholar]
  22. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  23. Seto J. T., Becht H., Rott R. 1974; Effect of specific antibodies on biological functions of the envelope components of Newcastle disease virus. Virology 61:354–360
    [Google Scholar]
  24. Shimizu Y. K., Shimizu K., Ishida N., Homma M. 1976; On the study of Sendai virus hemolysis. II.Morphological study of envelope fusion and hemolysis. Virology 71:48–60
    [Google Scholar]
  25. Terry G. M., Ho-Terry L. 1976; Fusion and haemolysis of chick erythrocytes by Newcastle disease virus. Archives of Virology 50:37–44
    [Google Scholar]
  26. Väänänen P., Kaariainen L. 1979; Haemolysis by two alphaviruses: Semliki Forest and Sindbis virus. Journal of General Virology 43:593–601
    [Google Scholar]
  27. Väänänen P., Vaheri A. 1971; Large-scale purification of rubella virus and preparation of an experimental split rubella virus vaccine. Applied Microbiology 22:255–259
    [Google Scholar]
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