1887

Abstract

SUMMARY

Two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of HSV-2-specific DNA sequences. Both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the HSV-2 genome. Revertant cells, which contained no detectable thymidine kinase, did not contain this DNA sequence. The failure of R1-restricted HSV-2 DNA to act as a donor of the thymidine kinase gene in transformation experiments suggests that the gene lies close to the R1 restriction site within this sequence at a map position of approx. 0.3. The HSV-2 kinase gene is therefore approximately co-linear with the HSV-1 gene.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-2-489
1979-11-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/2/JV0450020489.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-2-489&mimeType=html&fmt=ahah

References

  1. Bacchetti S., Graham F. 1977; Transfer of the gene for thymidine kinase to thymidine kinase deficient cells by purified herpes simplex viral DNA. Proceedings of the National Academy of Sciences of the United States of America 74:1590–1594
    [Google Scholar]
  2. Bacchetti S., Graham F. 1979; Characterisation of human TKr cell lines transformed to a TK+ phenotype by herpes simplex virus type 2 DNA. Journal of General Virology 42:149–157
    [Google Scholar]
  3. Commerford S. L. 1971; Iodination of nucleic acids in vitro . Biochemistry io:1993–2000
    [Google Scholar]
  4. Cortini R., Wilkie N. M. 1978; Physical maps for HSV-2 DNA with five restriction endonucleases. Journal of General Virology 39:259–280
    [Google Scholar]
  5. Davidson R. L., Adelstein S. J., Oxman M. N. 1973; Herpes simplex virus as a source of thymidine kinase for thymidine kinase deficient mouse cells: suppression and reactivation of the viral enzyme. Proceedings of the National Academy of Sciences of the United States of America 70:192–196
    [Google Scholar]
  6. Davis D. B., Kingsbury D. T. 1976; Quantitation of viral DNA present in cells transformed by U.V.-irradiated herpes simplex virus. Journal of Virology 17:783–788
    [Google Scholar]
  7. Kaufman E. R., Davidson R. L. 1975; Control of the expression of a herpes simplex virus thymidine kinase gene incorporated into thymidine kinase deficient mouse cells. Somatic Cell Genetics 1:153–164
    [Google Scholar]
  8. Kit S., Dubbs D. R. 1977; Regulation of herpes virus thymidine kinase activity in LM(TK) cells transformed by ultraviolet light-irradiated herpes simplex virus. Virology 76:331–340
    [Google Scholar]
  9. Kraizelburd E., Gage L. P., Weissbach A. 1975; Presence of a herpes simplex virus DNA fragment in an L-cell clone obtained after infection with irradiated herpes simplex virus. Journal of Molecular Biology 97:533–542
    [Google Scholar]
  10. Maitland N. J., Mcdougall J. K. 1977; Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA. Cell 11:233–241
    [Google Scholar]
  11. Minson A. C., Wildy P., Buchan A., Darby G. 1978; Introduction of the herpes simplex virus thymidine kinase into mouse cells using virus DNA or transformed cell DNA. Cell 13:581–587
    [Google Scholar]
  12. Minson A. C., Bastow K., Darby G. 1979; The herpes thymidine kinase gene as a transmissible genetic element in mammalian cells. In Antiviral Mechanisms in the Control of Neoplasia pp 7–16 Edited by Chandra P. New York: Plenum Press;
    [Google Scholar]
  13. Morse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of herpes simplex virus DNA. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  14. Munyon W., Kraizelburd E., Davis S., Mann J. 1971; Transfer of thymidine kinase to thymidine kinaseless L-cells by infection with ultra violet irradiated herpes simplex virus. Journal of Virology 7:813–820
    [Google Scholar]
  15. Sharp P. A., Petterson U., Sambrook J. 1974; Viral DNA in transformed cells. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. Journal of Molecular Biology 86:709–726
    [Google Scholar]
  16. Smith M. J., Britten R. J., Davidson E. H. 1975; Studies on nucleic acid re-association kinetics: reactivity of single strand tails in DNA-DNA renaturation. Proceedings of the National Academy of Sciences of the United States of America 72:4805–4809
    [Google Scholar]
  17. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  18. Steinberg B., Pollack R., Topp W., Botchan M. 1978; Isolation and characterisation of T-antigen negative revertants of a line of transformed rat cells containing one copy of the SV40 genome. Cell 13:19–32
    [Google Scholar]
  19. Stow N. D., Subak-Sharpe J. H., Wilkie N. M. 1978; Physical mapping of herpes simplex virus type i mutants by marker rescue. Journal of Virology 28:182–192
    [Google Scholar]
  20. Wigler M., Silverstein S., Lee L.-S., Pellicer A., Cheng Y.-C., Axel R. 1977; Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell 11:223–232
    [Google Scholar]
  21. Wigler M., Sweet R., Kee Sim G., Wold W., Pellicer A., Lacy E., Maniatis T., Silverstein S., Axel R. 1979; Transformation of mammalian cells with genes from procaryotes and eukaryotes. Cell 16:777–785
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-45-2-489
Loading
/content/journal/jgv/10.1099/0022-1317-45-2-489
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error