%0 Journal Article %A Minson, A. C. %A Darby, G. K. %A Wildy, P. %T Virus-specific DNA Sequences Present in Cells which Carry the Herpes Simplex Virus Thymidine Kinase Gene %D 1979 %J Journal of General Virology, %V 45 %N 2 %P 489-496 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-45-2-489 %I Microbiology Society, %X SUMMARY Two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of HSV-2-specific DNA sequences. Both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the HSV-2 genome. Revertant cells, which contained no detectable thymidine kinase, did not contain this DNA sequence. The failure of EcoR1-restricted HSV-2 DNA to act as a donor of the thymidine kinase gene in transformation experiments suggests that the gene lies close to the EcoR1 restriction site within this sequence at a map position of approx. 0.3. The HSV-2 kinase gene is therefore approximately co-linear with the HSV-1 gene. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-45-2-489