1887

Abstract

SUMMARY

The envelope spikes of Sindbis and Semliki Forest virus are arranged in a T=4 icosahedral surface lattice and, by deduction, it has been suggested that the nucleocapsid proteins are similarly arranged. After treatment of the virions with a non-ionic detergent the released nucleocapsids sediment in sucrose gradients at about 160S and 150S and have densities in CsCl of 1.42 g/ml and 1.425 g/ml, respectively, for Sindbis and Semliki Forest virus. At pH 6.0 Sindbis nucleocapsids do not contract like those of Semliki Forest virus. Nucleocapsids of both viruses are sensitive to the action of ribonuclease but only those of Semliki Forest virus undergo a drastic structural rearrangement due to the treatment. EDTA treatment in hypotonic conditions results in a decrease in the S-value for both particles. Electron micrographs show that the SFV nucleocapsids are partly ‘unfolded’ while those of Sindbis appear slightly contracted after exposure to EDTA.

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1979-10-01
2022-07-02
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References

  1. Bancroft J. B., Hiebert E., Rees M. W., Markham R. 1968; Properties of cowpea chlorotic mottle virus, its protein and nucleic acid. Virology 34:224–349
    [Google Scholar]
  2. Bonsdorff C.-H. Von 1972; Structural role of RNA in Semliki Forest virus nucleocapsid. Acta Pathologica et Microbiologica Scandinavica B80:579–588
    [Google Scholar]
  3. Bonsdorff C.-H. Von 1973; The structure of Semliki Forest virus. Commentationes Biologicae 74:1–53 (Societas Scientarium Fennica, Helsinki, Finland)
    [Google Scholar]
  4. Bonsdorff C.-H. Von, Harrison S. C. 1975; Sindbis virus glycoproteins form a regular icosahedral surface lattice. Journal of Virology 16:141–145
    [Google Scholar]
  5. Brady J. N., Winston V. D., Consigli R. A. 1977; Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions. Journal of Virology 23:717–724
    [Google Scholar]
  6. Brown F., Smale C. J., Horzinek M. C. 1974; Lipid and protein organization in vesicular stomatitis and Sindbis viruses. Journal of General Virology 22:455–458
    [Google Scholar]
  7. Burge B. W., Pfefferkorn E. R. 1966; Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology 30:214–223
    [Google Scholar]
  8. Butler P. J. G., Durham A. C. H., Klug A. 1972; Structures and roles of the polymorphic forms of tobacco mosaic virus protein. IV. Control of mode of aggregation of tobacco mosaic virus protein by protein binding. Journal of Molecular Biology 72:1–18
    [Google Scholar]
  9. Caspar D. L. D. 1963; Assembly and stability of the tobacco mosaic virus particle. Advances in Protein Chemistry 18:37–121
    [Google Scholar]
  10. Durham A. C. H., Haidar M. A. 1977; Cation binding by tobacco rattle virus. Virology 77:520–523
    [Google Scholar]
  11. Durham A. C. H., Hendry D. A. 1977; Cation binding by tobacco mosaic virus. Virology 77:510–519
    [Google Scholar]
  12. Durham A. C. H., Hendry D. A., Von Wechmar M. B. 1977; Does calcium ion binding control plant virus disassembly. Virology 77:524–533
    [Google Scholar]
  13. Garoff H., Simons K. 1974; Location of the spike glycoproteins in the Semliki Forest virus membrane. Proceedings of the National Academy of Sciences of the United States of America 71:3988–3992
    [Google Scholar]
  14. Garoff H., Simons K., Renkonen O. 1974; Isolation and characterization of the membrane proteins of Semliki Forest virus. Virology 61:493–504
    [Google Scholar]
  15. Harrison S. C., David A., Jumblatt J., Darnell J. E. 1971; Lipid and protein organization in Sindbis virus. Journal of Molecular Biology 60:523–528
    [Google Scholar]
  16. Harrison S. C., Jack A., Goodenough D., Sefton B. M. 1974; Structural studies of spherical viruses. Journal of Supramolecular Structure 2:486–495
    [Google Scholar]
  17. Horzinek M. C. 1973; The structure of togaviruses. Progress in Medical Virology 16:109–156
    [Google Scholar]
  18. Hsu C. H., Seghal O. P., Pickett E. E. 1976; Stabilizing effects of divalent metal ions on virions of southern bean mosaic virus. Virology 69:587–595
    [Google Scholar]
  19. Hull R. 1977; The stabilization of the particles of turnip rosette virus and other members of the Southern bean mosaic virus group. Virology 79:58–66
    [Google Scholar]
  20. Incardona N. L., Kaesberg P. 1964; A pH induced structural change in bromegrass mosaic virus. Biophysical Journal 4:11–21
    [Google Scholar]
  21. Incardona N. L., Blonski R., Feeney W. 1972; Mechanism of adsorption and eclipse of bacteriophage ϕ X 174. In vitro conformational change under conditions of eclipse. Journal of Virology 9:96–101
    [Google Scholar]
  22. Jacrot B. 1975; Studies on the assembly of a spherical plant virus. II. The mechanism of protein aggregation and virus swelling. Journal of Molecular Biology 95:433–446
    [Google Scholar]
  23. Kääriäinen L., Söderlund H. 1971; Properties of Semliki Forest virus nucleocapsid: I. Sensitivity to pancreatic ribonuclease. Virology 43:291–299
    [Google Scholar]
  24. Kääriäinen L., Söderlund H. 1978; Structure and replication of alphaviruses. Current Topics of Microbiology and Immunology 82:16–57
    [Google Scholar]
  25. Kääriäinen L., Simons K., Von Bonsdorff C.-H. 1969; Studies in subviral components of Semliki Forest virus. Annales Medicinae Experimentalis et Biologiae Fenniae 47:235–248
    [Google Scholar]
  26. Kaper J. M. 1971; Studies on the stabilizing forces of simple RNA viruses. I. Selective interference with protein-RNA interactions in turnip yellow mosaic virus. Journal of Molecular Biology 56:259–279
    [Google Scholar]
  27. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  28. Pfefferkorn E. R., Clifford R. L. 1963; Precipitation and recovery of Sindbis virus from solutions of low ionic strength. Virology 21:273–274
    [Google Scholar]
  29. Pfefferkorn E. R., Shapiro D. 1974; Reproduction of togaviruses. In Comprehensive Virology vol 2: pp 171–230 Edited by Fraenkel-Conrat H. Wagner R. R. New York and London: Plenum Press;
    [Google Scholar]
  30. Sefton B. M., Keegstra K. 1974; Glycoproteins of Sindbis virus: preliminary characterization of the oligosaccharides. Journal of Virology 14:522–530
    [Google Scholar]
  31. Söderlund H., Kääriäinen L., Von Bonsdorff C.-H., Weckström P. 1972; Properties of Semliki Forest virus nucleocapsid: II. An irreversible contraction by acid pH. Virology 47:753–760
    [Google Scholar]
  32. Söderlund H., Glanville N., Kääriäinen L. 1973/74; Polysomal RNAs in Semliki Forest virus-infected cells. Intervirology 2:100–113
    [Google Scholar]
  33. Söderlund H., Kääriäinen L., Von Bonsdorff C.-H. 1975; Properties of Semliki Forest virus nucleocapsid. Medical Biology 53:412–417
    [Google Scholar]
  34. Strauss J. H., Strauss E. G. 1976; Togaviruses. In The Molecular Biology of Animal Viruses pp 111–166 Edited by Nayak D. P. New York: Marcel Dekker;
    [Google Scholar]
  35. Tuomi K., Kääriäinen L., Söderlund H. 1975; Quantitation of Semliki Forest virus RNAs in infected cells using 32P equilibrium labelling. Nucleic Acids Research 2:555–565
    [Google Scholar]
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