1887

Abstract

Summary

The DNA of nuclear polyhedrosis virus (AcNPV) was isolated from extracellular non-occluded virions. The guanosine plus cytosine content of AcNPV DNA was estimated from its buoyant density in CsCl and its T value to be about 43 mol %. In ethidium bromide-CsCl gradients double-stranded covalently closed DNA molecules (form I) were detected, while relaxed circular (form II) and linear (form III) DNA molecules were demonstrated by velocity sedimentation in sucrose. The mol. wt. of AcNPV DNA was calculated to be about 78 × 10 from the sedimentation value of form III DNA. Digestion of AcNPV DNA with restriction endonucleases RI and I resulted in 21 and 7 fragments, respectively, after analysis on agarose gels. The mol. wt. of the fragments and their molar ratio were determined. By this method the mol. wt. of AcNPV DNA was calculated to be about 83 × 10.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-2-333
1979-08-01
2019-10-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-2-333
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error