1887

Abstract

SUMMARY

The ability of herpes simplex virus type 1 to replicate in cells transformed by adenovirus type 5 is strongly dependent on the origin of the cells. Studies show that adenovirus transformed rat cells lose their permissiveness while cells of hamster or human origin retain their ability to replicate HSV although at a reduced level when compared to the untransformed parent cells. One line of adenovirus transformed rat cells, 107, demonstrates thermosensitive events, allowing HSV to replicate at 34 °C but not at 37 °C. Analysis of the biochemical events taking place at 37 °C showed that virus-specific DNA synthesis was greatly reduced but that all of the late virus structural proteins could be observed after SDS-polyacrylamide gel electrophoresis. It was also demonstrated that shut-off of host macromolecular synthesis appeared to be less efficient after HSV infection of 107 cells than after infection of more permissive cells such as the non-transformed REF line. Collectively the data show that interactions between HSV and the host cell are perturbed when the cell is transformed by type 5 adenovirus. The degree of perturbation ranges from a slight reduction in number of progeny to a completely abortive infection.

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/content/journal/jgv/10.1099/0022-1317-44-2-297
1979-08-01
2024-12-06
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References

  1. Aurelian L., Roizman B. 1965; Abortive infection of canine cells by herpes simplex virus II. Alternative suppression of synthesis of interferon and viral constituents. Journal of Molecular Biology 11:539–548
    [Google Scholar]
  2. Cohen O. H., Vauohan R. K., Lawrence W. C. 1971; Deoxyribonucleic acid synthesis in synchronized mammalian KB cells infected with herpes simplex virus. Journal of Virology 7:783–791
    [Google Scholar]
  3. Darlington R. W., Granoff A. 1973; Replication-biological aspects. In The Herpesviruses chapter 4, pp 93–132 Edited by Kaplan A. S. London and New York: Academic Press;
    [Google Scholar]
  4. Dochertv J. J., Mantyjarvi R. A., Rapp F. 1972; Mechanism of the restricted growth of herpes simplex virus type 2 in a hamster cell line. Journal of General Virology 16:255–264
    [Google Scholar]
  5. Doller E., Duff R., Rapp F. 1973; Resistance of hamster cells transformed by herpes simplex virus type 2 to superinfection by herpes simplex virus. Intervirology 1:154–167
    [Google Scholar]
  6. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science 130:432–437
    [Google Scholar]
  7. Flanagan J. F. 1967; Virus specific ribonucleic acid synthesis in KB cells infected with herpes simplex virus. Journal of Virology 1:583–590
    [Google Scholar]
  8. Freeman A. E., Black P. H., Wolford R., Huebner R. J. 1967; Adenovirus-type 12 rat embryo transformation system. Journal of Virology 1:362–367
    [Google Scholar]
  9. Geder L., Vaczi L., Jeney E. 1971; Studies on restricted growth of herpes viruses in SV-40 transformed cell lines. Acta Virologica 15:35–46
    [Google Scholar]
  10. Graham F. L., Abrahams P. J., Mulder C., Heijneker H. L., Warnaar S. O., Devries F. A. J., Fiers W., van De Reb A. J. 1974; Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and Simian virus 40. Cold Spring Harbor Symposium on Quantitative Biology 39:637–650
    [Google Scholar]
  11. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
    [Google Scholar]
  12. Honess R. W., Roizman B. 1973; Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and non-structural herpes virus polypeptides in the infected cell. Journal of Virology 12:1347–1368
    [Google Scholar]
  13. Honess R. W., Roizman B. 1974; Regulation of herpes virus macro-molecular synthesis. I. Cascade regulation of the synthesis of three groups of viral polypeptides. Journal of Virology 14:8–19
    [Google Scholar]
  14. Koment R. W., Rapp F. 1975; Variation in susceptibility of different cell types to temperature-sensitive host range mutants of herpes simplex virus type 2. Virology 64:164–169
    [Google Scholar]
  15. Maizel J. V. Jun 1971; Polyacrylamide gel electrophoresis of viral proteins. In Methods in Virology volume 5 pp 197–246 Edited by Maramorosch K., Koprowski H. London and New York: Academic Press;
    [Google Scholar]
  16. Nordaa J., van Der Enders J. F., Diamandopoulos G. 1966; Increased resistance to herpes simplex virus of hamster and human cells transformed by SV-40. Proceedings of Society for Experimental Biology and Medicine 122:915–920
    [Google Scholar]
  17. Rapp F., Trulock S. C. 1970; Susceptibility to superinfection of Simian cells transformed by SV-40. Virology 40:961–970
    [Google Scholar]
  18. Roizman B., Furlong D. 1974; The replication of Herpesviruses. In Comprehensive Virology vol 3 pp 229–403 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  19. Sambrook J., Botchan M., Gallimore P., Ozanne B., Pettersson U., Williams J., Sharp P. 1974; Viral DNA sequences in cells transformed by Simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harbor Symposium on Quantitative Biology 39:615–632
    [Google Scholar]
  20. Spector D., Pizer L. 1978; Herpes virus infection modifies adenovirus RNA metabolism in adenovirus type 5 transformed cells. Journal of Virology 27:1–12
    [Google Scholar]
  21. Sprecher-Goldberger S., Lefebvre N., Debruyne J., Pochet R., Thiry L. 1973; Thermosensitive events in adenovirus transformed cells. I. Resistance to herpes simplex virus. Archiv fur die gesamte Virus-forschung 40:236–247
    [Google Scholar]
  22. Tucker A., Docherty J. 1975; Adenovirus transformed cells restrict herpes simplex virus replication. Infection and Immunity 11:556–562
    [Google Scholar]
  23. Yanagi K., Talavera A., Nishimoto T., Rush M. 1978; Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants. Journal of Virology 25:42–50
    [Google Scholar]
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