1887

Abstract

SUMMARY

Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.

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/content/journal/jgv/10.1099/0022-1317-43-3-633
1979-06-01
2024-05-13
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References

  1. Adams J. M., Imagawa D. T. 1962; Measles antibodies in multiple sclerosis. Proceedings of the Society for Experimental Biology and Medicine m:562–566
    [Google Scholar]
  2. Burridge K. 1976; Changes in cellular glycoproteins after transformation: identification of specific glycoproteins and antigens in sodium dodecyl sulphate gels. Proceedings of the National Academy of Sciences of the United States of America 73:4457–4461
    [Google Scholar]
  3. Hall W. W., Martin S. J. 1973; Purification and characterization of measles virus. Journal of General Virology 19:175–188
    [Google Scholar]
  4. Hardwick J. M., Bussell R. H. 1978; Glycoproteins of measles virus under reducing and nonreducing conditions. Journal of Virology 25:687–692
    [Google Scholar]
  5. Horta-Barbosa L., Fuccillo D. A., London W. T., Jarbour J. T., Zemen W., Sever J. L. 1969; Isolation of measles virus from brain cell cultures of two patients with subacute sclerosing panencephalitis. Proceedings of the Society for Experimental Biology and Medicine 132:272–277
    [Google Scholar]
  6. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specificity activity. Nature, London 194:495–496
    [Google Scholar]
  7. Katz S. L., Enders J. F. 1969; Measles virus. In Diagnostic Procedures for Viral and Rickettsial infections pp. 504–528 Edited by Lennette E. H., Schmidt N. I. New York: American Public Health Association Inc;
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Lamb R. A., Mahy W. I., Choppin P. W. 1976; The synthesis of Sendai virus polypeptides in infected cells. Virology 69:116–131
    [Google Scholar]
  10. Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. McFarlin D. E., Johnson J. S., Seymore W. F. K. 1968; Antimuscle factor and antinuclear factor in patients with myasthenia gravis. I. Heavy chain determinants. Journal of Immunology 101:104–110
    [Google Scholar]
  12. Mountcastle W. E., Choppin P. W. 1977; A comparison of the polypeptides of four measles virus strains. Virology 78:463–474
    [Google Scholar]
  13. Seifried A. S., Aalbrecht P., Milstien J. B. 1978; Characterization of an RNA-dependent RNA polymerase activity associated with measles virus. Journal of Virology 25:781–787
    [Google Scholar]
  14. Stumph W. E., Elgin S. C. R., Hood L. 1974; Antibodies to proteins dissolved in sodium dodecyl sulphate. Journal of Immunology 13:1752–1756
    [Google Scholar]
  15. Tyrrell D. L. J., Norrby E. 1978; Structoral polypeptides of measles virus. Journal of General Virology 39:219–229
    [Google Scholar]
  16. Wang E., Wolf B. A., Lamb R. A., Choppin P. W., Goldberg A. R. 1976; The presence of actin in enveloped viruses. In Cold Spring Harbor Conference on Cell Motility Cold Spring Harbor Laboratory; 3589–59
    [Google Scholar]
  17. Wechsler S. L., Fields B. N. 1978; Intracellular synthesis of measles virus-specified polypeptides. Journal of Virology 25:285–297
    [Google Scholar]
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