1887

Abstract

SUMMARY

Simian rotavirus (SA-11) isolated from infected African green monkey kidney cells was separated into two virus fractions in a CsCl density gradient and their proteins analysed on a continuous phosphate buffered polyacrylamide gel electrophoresis system. One peak (buoyant density 1.37 g/ml) contained double capsid virus particles which were radioimmunoassay (RIA)- and haemagglutinin (HA)-positive and yielded eight polypeptides whose mol. wt. ranged from 48000 to 128000. The second peak (buoyant density 1.39 g/ml) which contained 70% single capsid particles and was RIA-positive but HA-negative, yielded only five polypeptides. The three polypeptides missing in the second peak are associated presumably with the outer capsid of SA-11 virus particles and one or more of these is assumed to be the HA of SA-11 rotavirus.

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/content/journal/jgv/10.1099/0022-1317-43-2-463
1979-05-01
2024-05-14
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References

  1. Bridger J. C., Woode G. N. 1976; Characterization of two particle types of calf rotavirus. Journal of General Virology 31:245–250
    [Google Scholar]
  2. Kalica A. R., Garon C. F., Wyatt R. G., Mebus C. A., van kirk D. H., Chanock R. M., Kapikian A. Z. 1976; Differentiation of human and calf reovirus-like agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA. Virology 74:86–92
    [Google Scholar]
  3. Kalica R. R., James H. D. Jun, Kapikian A. Z. 1978a; Hemagglutination by simian rotavirus. Journal of Clinical Microbiology 7:314–315
    [Google Scholar]
  4. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Chanock R. M., Kapikian A. Z. 1978b; Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247–255
    [Google Scholar]
  5. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  6. Maizel J. V. Jun 1971; Polyacrylamide gel electrophoresis of viral protein. In Methods in Virology vol 5 pp 177–246 Edited by Maramorosch K., Koprowski H. New York: Academic Press;
    [Google Scholar]
  7. Newman J. F. E., Brown F., Bridger J. C., Woode G. N. 1975; Characterization of a rotavirus. Nature, London 258:631–633
    [Google Scholar]
  8. Obijeski J. F., Palmer E. L., Martin M. L. 1977; Biochemical characterization of infantile gastroenteritis virus (IGV). Journal of General Virology 34:485–497
    [Google Scholar]
  9. Rodger S. M., Schnagl R. D., Holmes I. H. 1975; Biochemical and biophysical characterization of diarrhea viruses of human and calf origin. Journal of Virology 16:1229–1235
    [Google Scholar]
  10. Rodger S. M., Schnagl R. D., Holmes I. H. 1977; Further biochemical characterization, including the detection of surface glycoproteins, of human calf and simian rotaviruses. Journal of Virology 24:91–98
    [Google Scholar]
  11. Schnagl R. D., Holmes I. H. 1976; Characteristics of the genome of human infantile enteritis virus (rotavirus). Journal of Virology 19:267–270
    [Google Scholar]
  12. Todd D., Mcnulty M. S. 1977; Biochemical studies on a reovirus-like agent (rotavirus) from lambs. Journal of Virology 21:1215–1218
    [Google Scholar]
  13. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecylsulfate-poly-acrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
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