1887

Abstract

SUMMARY

The continuous culture of a hamster melanoma cell line has led to the spontaneous appearance of a retrovirus (HaRV) with typical type-C characteristics. The virus differs from all other known hamster viruses in its ability to transform murine as well as rat and hamster cells with apparent one-hit kinetics. Guinea pig, human and feline cells were not transformed although reverse transcriptase activity was detected in the supernatant from infected human cells. HaRV-transformed hamster embryo cells produced solid tumours (all non-pigmented) in 4 out of 35 animals when injected into hamsters while HaRV-transformed murine cells produced no tumours in mice. Injection of HaRV alone in hamsters, mice and rabbits did not induce tumours. HaRV possesses a 70S RNA which dissociates to 35S in DMSO and has a reverse transcriptase which utilizes the 70S virus RNA as a template. The size, morphology and density (1.15 g/ml) are similar to other known type-C viruses. Polyacrylamide gel electrophoresis indicates the presence of polypeptides analogous to those found in other type-C viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-2-317
1979-05-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/2/JV0430020317.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-2-317&mimeType=html&fmt=ahah

References

  1. Aaronson S. A., Stephenson J. R. 1976; Endogenous type-C RNA viruses of mammalian cells. Biochimica et Biophysica Acta 458:324–354
    [Google Scholar]
  2. Aaronson S. A., Weaver C. A. 1971; Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. Journal of General Virology 13:245–252
    [Google Scholar]
  3. Aaronson S. A., Hartley J. W., Todaro G. J. 1969; Mouse leukemia virus: ‘spontaneous’ release by mouse embryo cells after long term in vitro cultivation. Proceedings of the National Academy of Sciences of the United States of America 65:87–94
    [Google Scholar]
  4. Aaronson S. A., Jainchill J. C., Todaro G. J. 1970; Murine sarcoma virus transformation of BaLB/3T3 cells: lack of dependence on murine leukemia virus. Proceedings of the National Academy of Sciences of the United States of America 66:1236–1243
    [Google Scholar]
  5. Bader J. P., Steck T. L. 1969; Analysis of the ribonucleic acid of murine leukemia virus. Journal of Virology 4:454–459
    [Google Scholar]
  6. Barbacid M., Tronick S. R., Aaronson S. A. 1978; Isolation and characterization of an endogenous type C RNA virus of mink (MvILV) cells. Journal of Virology 25:129–137
    [Google Scholar]
  7. Bassin R. H., Simons P. J., Chesterman F. C., Harvey J. J. 1968; Murine sarcoma virus (Harvey): characteristics of focus formation in mouse embryo cell cultures and virus production by hamster tumor cells. International Journal of Cancer 3:265–272
    [Google Scholar]
  8. Charman H. P., Kin N., Gilden R. V. 1974; Radioimmunoassay for the major structural protein of hamster type-C viruses. Journal of Virology 14:910–917
    [Google Scholar]
  9. Darling T., Albert J., Albert D., Reid T. 1977; Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns. Journal of Chromatography 131:383–390
    [Google Scholar]
  10. Gregerson D. S., Russell P., Reid T. W. 1979; Biochemical and immunological properties of the reverse transcriptase associated with a hamster retrovirus. Journal of General Virology (following paper)
    [Google Scholar]
  11. Hartley J. W., Rowe W. P. 1966; Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proceedings of the National Academy of Sciences of the United States of America 55:780–786
    [Google Scholar]
  12. Huebner R. J., Kelloff G. F., Sarma P. S., Lane W. T., Turner H. C., Gilden R. V., Oroszlan S., Meier H., Myers D. D., Peters R. L. 1970; Group-specific antigen expression during embryogenesis of the genome of the C-type RNA tumor virus: implication for ontogenesis and oncogenesis. Proceedings of the National Academy of Sciences of the United States of America 67:366–376
    [Google Scholar]
  13. Kelloff G., Huebner R. J., Lee Y. K., Toni R., Gilden R. 1970; Hamster-tropic sarcomagenic and non-sarcomagenic viruses derived from hamster tumors induced by the Gross pseudotype of Moloney sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 65:310–317
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409–414
    [Google Scholar]
  16. O’Connor T. E., Fischinger P. J. 1968; Titration patterns of a murine sarcoma-leukemia virus complex: evidence for existence of competent sarcoma virions. Science 159:325–329
    [Google Scholar]
  17. O’Connor T. E., Fischinger P. J. 1969; Physical properties of competent and defective states of a murine sarcoma (Moloney) virus. Journal of the National Cancer Institute 43:487–497
    [Google Scholar]
  18. Okabe H., Gilden R. V., Hatanaka M. 1974; Specificity of the DNA product of RNA-dependent DNA polymerase in type-C viruses. III. Analysis of viruses derived from Syrian hamsters. Proceedings of the National Academy of Sciences of the United States of America 71:3278–3282
    [Google Scholar]
  19. Pincus T., Hartley J. W., Rowe W. P. 1975; A major genetic locus affecting resistance to infection with murine leukemia viruses. IV. Dose-response relationships in Fv-1-sensitive and resistant cell cultures. Virology 65:333–342
    [Google Scholar]
  20. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–215
    [Google Scholar]
  21. Schlom J., Spiegelman S. 1971; Simultaneous detection of reverse transcriptase and high molecular weight RNA unique to oncogenic RNA viruses. Science 174:840–843
    [Google Scholar]
  22. Somers K. D., May J. T., Kit S., McCormick K. J., Hatch G. G., Stenback W. A., Trentin J. J. 1973; Biochemical properties of a defective hamster C-type oncornavirus. Intervirology 1:11–18
    [Google Scholar]
  23. Strand M., August J. T. 1974; Structural proteins of RNA tumor viruses as probes for viral gene expression. Cold Spring Harbor Symposia on Quantitative Biology 39:1109–1116
    [Google Scholar]
  24. Verma I. M., Meuth N. L., Fan H., Baltimore D. 1974; Hamster leukemia virus: lack of endogenous DNA synthesis and unique structure of its DNA polymerase. Journal of Virology 13:1075–1082
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-2-317
Loading
/content/journal/jgv/10.1099/0022-1317-43-2-317
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error