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Volume 4, Issue 2

Rod-shaped Pyocin 28 Free

Abstract

Summary

Pyocin 28 was obtained by induction of 28 with mitomycin. Pyocin activity was correlated with the number of rod-shaped particles in purified preparations. The width of the pyocin rod was uniform, measuring about 90 Å, but the length was not uniform, varying from 200 to 4000 Å, but rods measuring 1000 to 1200 Å were most frequent. A dark central line and regular cross-striations were usually seen on the rod, and a fine fibre was sometimes visible at the sharp end. Pyocin activity was slightly reduced from ultrasonic treatment, but not at all by trypsin, Nagarse, DNase and RNase. The pyocin was stable between pH 5.0 and 8.0, and was completely inactivated by heating at 60° for 10 min. Specific attachment of numerous pyocin rods to the surface of sensitive bacteria was observed by the electron microscope.

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© Journal of General Virology 1969
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1969-03-01
2024-05-14
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References

  1. Bradley D. E. 1964; The structure of some bacteriophages associated with male strains of Escherichia coli. J. gen. Microbiol 35:471
     [Google Scholar]
  2. Bradley D. E. 1967; Ultrastructure of phages and bacteriocins. Bad. Rev 31:230
     [Google Scholar]
  3. Bradley D. E., Dewar C. A. 1966; The structure of phagelike objects associated with non-induced bacteriocinogenic bacteria. J. gen. Microbiol 45:399
     [Google Scholar]
  4. Coetzee H. L., De Klerk H. C., Coetzee J. N., Smit J. A. 1968; Bacteriophage tail-like particles associated with intra-species killing of. Proteus vulgaris. J. gen. Virol 2:29
     [Google Scholar]
  5. Eserling F. A., Boy de la Tour E. 1965; Capsomeres and other structures observed on some bacteriophages. Pathologia Microbiol 28:175
     [Google Scholar]
  6. Endo H., Ayabe K., Amako K., Takeya K. 1965; Inducible phage of. Escherichia coli 15. Virology 25:469
     [Google Scholar]
  7. Higerd T. B., Baechler C. A., Berk R. S. 1967; In vitro and in vivo characterization of pyocin. J. Bacterial 93:1976
     [Google Scholar]
  8. Ishn Y., Nism Y., Egami F. 1965; The fine structure of a pyocin. J. molec. Biol 13:428
     [Google Scholar]
  9. Lang D., McDonald T. O., Gardner E. W. 1968; Electron microscopy of particles associated with a bacteriocinogenic Vibrio cholerae strain. J. Bad 95:708
     [Google Scholar]
  10. Meningmann H. D. 1965; Electronmicroscopy of the antibacterial agent produced by. Escherichia coli 15. J. gen. Microbiol 41:151
     [Google Scholar]
  11. Sandoval H. K., Reilly C. H., Tandler B. 1965; Colicin 15: possibly a defective bacteriophage. Nature, Lond 205:522
     [Google Scholar]
  12. Slayter H. S., Holloway B. W., Hall C. E. 1964; The structure of Pseudomonas aeruginosa phages, B3, E79, and F116. J Ultrastrud. Res. 11:274
     [Google Scholar]
  13. Takeya K., Amako K. 1964; The structure of mycobacteriophages. Virology 24:461
     [Google Scholar]
  14. Takeya K., Amako K. 1966; A rod-shaped Pseudomonas phage. Virology 28:163
     [Google Scholar]
  15. Takeya K., Minamishima Y., Amako K., Ohnishi Y. 1966; A small rod-shaped pyocin. Virology 31:167
     [Google Scholar]
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Rod-shaped Pyocin 28
J. Gen. Virol. 4, 145 (1969); https://doi.org/10.1099/0022-1317-4-2-145
/content/journal/jgv/10.1099/0022-1317-4-2-145
/content/journal/jgv/10.1099/0022-1317-4-2-145
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