1887

Abstract

SUMMARY

Serum obtained from mice 3 to 5 weeks after the third i.p. dose of dengue type 2 virus (DV) protected recipient mice against intracerebral challenge with DV, whereas the serum obtained after 1 and 2 weeks provided minimum protection. Adoptive intravenous transfer of immune spleen cells obtained from mice 1 to 5 weeks after immunization did not protect recipient mice against even a small dose (10 LD) of DV. Depletion of T-cells by treatment of mice with anti-thymocyte serum did not potentiate DV infection. Development of a cell-mediated immune response (CMI) against DV was noted only at two periods by the leucocyte migration inhibition test (LMI), with borderline values of 20 and 21%. Dengue virus did not cause illness or death in mice when given by i.p. or i.v. routes and this was not affected by pre-treatment of mice with silica to damage local macrophages. It is concluded that humoral antibody plays a critical role in recovery from primary dengue virus infection of mice whereas CMI and macrophages appear to have no protective role.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-2-293
1978-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/2/JV0390020293.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-2-293&mimeType=html&fmt=ahah

References

  1. Agrawal D. K., Tandon P., Chaturvedi U. C., Kumar A. 1978; Biochemical study of certain enzymes and metabolites of carbohydrate metabolism in the skeletal muscles of dengue virus infected mice. Journal of General Virology (in the press)
    [Google Scholar]
  2. Albrecht P. 1968; Pathogenesis of neurotropic arbovirus infections. Current Topics in Microbiology and Immunology 43:44–91
    [Google Scholar]
  3. Allison A. C. 1972; Immunity against viruses. In The Scientific Basis of Medicine Annual Review pp 49–73
    [Google Scholar]
  4. Allison A. C., Harington J. S., Birbeck M. 1966; An examination of cytotoxic effects of silica on macrophages. Journal of Experimental Medicine 124:141–153
    [Google Scholar]
  5. Aung-Khin M., Khin Ma. Ma, Thant Zin., Tinu M. 1975; Changes in the tissues of the immune system in dengue haemorrhagic fever. Journal of Tropical Medicine and Hygiene 78:256–261
    [Google Scholar]
  6. Bhamarapravati N., Toochinda P., Boonyapakanavik V. 1967; Pathology of Thailand haemorrhagic fever: a study of 100 autopsy cases. Annals of Tropical Medicine and Parasitology 61:500–510
    [Google Scholar]
  7. Blanden R. V. 1970; Mechanism of recovery from a generalised viral infection mouse-pox. I. The effect of antithymocyte serum. Journal of Experimental Medicine 132:1035–1054
    [Google Scholar]
  8. Camenga D. L., Nathanson N., Cole G. A. 1974; Cyclophosphamide – potentiated West Nile virus encephalitis: relative influence of cellular and hi:mo:al factors. Journal of Infectious Diseases 130:634–641
    [Google Scholar]
  9. Chaturvedi U. C., Kapoor A. K., Mathur A., Chandra D., Khan A. M., Mehrotra R. M. L. 1970a; A clinical and epidemiological study of an epidemic of febrile illness with haemorrhagic manifestations which occurred at Kanpur, India in 1968. Bulletin of the World Health Organization 43:281–287
    [Google Scholar]
  10. Chaturvedi U. C., Mathur A., Kapoor A. K., Mehrotra N. K., Mehrotra R. M. L. 1970b; Virological study of an epidemic of febrile illness with haemorrhagic manifestations at Kanpur, India during 1968. Bulletin of the World Health Organization 43:289–293
    [Google Scholar]
  11. Chaturvedi U. C., Mathur A., Mehrotra R. M. L. 1974; Experimentally produced cardiac injury following dengue virus infection. Indian Journal of Pathology and Bacteriology 17:218–220
    [Google Scholar]
  12. Chaturvedi U. C., Tandon P., Mathur A. 1977; Effect of immunosuppression on dengue virus infection in mice. Journal of General Virology 36:449–458
    [Google Scholar]
  13. Chaturvedi U. C., Tandon H. O., Mathur A. 1978; Control of in vitro spread of Coxsackie B4 virus infection by sensitized spleen cells and antibody. Journal of Infectious Diseases 138: (in the press)
    [Google Scholar]
  14. Clarke D. H., Casals J. 1958; Techniques for haemagglutination and haemagglutination inhibition with arthropod borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  15. Cole G. A., Wisseman C. L. Jun 1969; Pathogenesis of type 1 dengue virus infection in suckling, weanling and adult mice. I. The relation of virus replication to interferon and antibody formation. American Journal of Epidemiology 89:669–680
    [Google Scholar]
  16. David J. R., Al-Askarj S., Lawrence H. S., Thomas L. 1964; Delayed hypersensitivity in vitro. I. The specificity of inhibition of cell migration by antigens. Journal of Immunology 93:264–273
    [Google Scholar]
  17. Golljb E. S. 1971; Brain-associated theta antigen: reactivity of rabbit antimouse brain serum with mouse lymphoid cells. Cellular Immunology 2:353–361
    [Google Scholar]
  18. Halstead S. B. 1966; Mosquito-borne haemorrhagic fevers of South & South-East Asia. Bulletin of the World Health Organization 35:3–15
    [Google Scholar]
  19. Halstead S. B., Chow J. S., Marchette N. J. 1973; Immunological enhancement of dengue virus replication. Nature, New Biology 243:24–26
    [Google Scholar]
  20. Hammon W. M. 1966; Immunological response: possible role of human response as an etiological factor. Bulletin of the World Health Organization 25:55–56
    [Google Scholar]
  21. Hisrch M. S., Zisman B., Allison A. C. 1970; macrophages and age dependent resistance to herpes simplex virus in mice. Journal of Immunology 104:1160–1165
    [Google Scholar]
  22. Jerne N. K., Nordin A. A. 1963; Plaque formation in agar by single antibody producing cells. Science 140:405–406
    [Google Scholar]
  23. Kapoor A. K., Chaturvedi U. C., Tandon H. O., Mathur A. 1978; Three year study of dengue fever epidemics at Kanpur, India during 1968 to 1970. Journal of Hygiene (Cambridge) (in the press)
    [Google Scholar]
  24. Levey R. H., Medawar P. B. 1966; Nature and mode of action of antilymphocytic antiserum. Proceedings of the National Academy of Sciences of the United States of America 56:1130–1137
    [Google Scholar]
  25. Marchette N. J., Halstead S. B., Chow J. S. 1976; Replication of dengue viruses in cultures of peripheral blood leucocytes from Dengue immune rhesus monkeys. Journal of Infectious Diseases 133:274–282
    [Google Scholar]
  26. Nathanson N., Cole G. A. 1970; Immunosuppression and experimental virus infection of the nervous system. Advances in Virus Research vol 16 pp 397–448 London: Academic Press;
    [Google Scholar]
  27. Oritz-Oritz L., Zamacona G., Garmilla C., Arellano M. T. 1974; Migration inhibition test in leucocytes from patients allergic to penicillin. Journal of Immunology 113:993–997
    [Google Scholar]
  28. Pang T., Blanden R. V. 1976; Regulation of the T-cell response to Ectromelia virus infection. I. Feedback suppression by effector T-cells. Journal of Experimental Medicine 143:469–481
    [Google Scholar]
  29. Rager-Zisman B., Allison A. C. 1973; The role of antibody and host cells in the resistance of mice against infection by Coxsackie B3 virus. Journal of General Virology 19:329–338
    [Google Scholar]
  30. Rao T. R., Anderson C. R. 1964; Concluding review on the papers on the outbreak of febrile illness with haemorrhagic manifestations in Calcutta. Indian Journal of Medical Research 52:727–734
    [Google Scholar]
  31. Reed L. V., Muench H. J. 1938; A simple method of estimating fifty per cent end points. American Journal of Hygiene 27:493–497
    [Google Scholar]
  32. Tandon P., Chaturvedi U. C. 1977; Antibody forming cell response of mice to dengue virus given by different routes. Current Science 46:43–44
    [Google Scholar]
  33. Van Der Groen G., Van Den Berghe D. A. R., Pattyn S. R. 1977; Interaction of mouse peritoneal macrophages with different arboviruses in vitro. Journal of General Virology 34:353–361
    [Google Scholar]
  34. Zisman B., Wheelock E. F., Allison A. C. 1971; Role of macrophages and antibody in resistance of mice against yellow fever virus. Journal of Immunology 107:236–243
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-2-293
Loading
/content/journal/jgv/10.1099/0022-1317-39-2-293
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error