RT Journal Article SR Electronic(1) A1 Chaturvedi, U. C. A1 Tandon, Pushpa A1 Mathur, Asha A1 Kumar, A.YR 1978 T1 Host Defence Mechanisms Against Dengue Virus Infection of Mice JF Journal of General Virology, VO 39 IS 2 SP 293 OP 302 DO https://doi.org/10.1099/0022-1317-39-2-293 PB Microbiology Society, SN 1465-2099, AB SUMMARY Serum obtained from mice 3 to 5 weeks after the third i.p. dose of dengue type 2 virus (DV) protected recipient mice against intracerebral challenge with DV, whereas the serum obtained after 1 and 2 weeks provided minimum protection. Adoptive intravenous transfer of immune spleen cells obtained from mice 1 to 5 weeks after immunization did not protect recipient mice against even a small dose (10 LD50) of DV. Depletion of T-cells by treatment of mice with anti-thymocyte serum did not potentiate DV infection. Development of a cell-mediated immune response (CMI) against DV was noted only at two periods by the leucocyte migration inhibition test (LMI), with borderline values of 20 and 21%. Dengue virus did not cause illness or death in mice when given by i.p. or i.v. routes and this was not affected by pre-treatment of mice with silica to damage local macrophages. It is concluded that humoral antibody plays a critical role in recovery from primary dengue virus infection of mice whereas CMI and macrophages appear to have no protective role., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-39-2-293