1887

Abstract

SUMMARY

Experiments have been performed to identify the type and size of cell infected by EB virus in the blood of acute infectious mononucleosis (IM) patients, and to investigate the nature of the infection. Virus-infected cells, recognized by their ability to give rise to lymphoblastoid cell lines when co-cultivated with foetal lymphocytes, were shown to be restricted to the B-lymphocyte population. Samples of this population from each of eight IM patients were found to be negative for EB nuclear antigen (EBNA) staining. Thereafter, fractions of IM B-lymphocytes prepared on the basis of cell size were assayed either by co-cultivation, for the incidence of virus-infected cells, or by immunofluorescence staining for the presence of cells expressing EBNA. The great majority of virus-infected cells were found in the fractions of normal sized B-lymphocytes and yet these fractions were unequivocally EBNA-negative. The finding of EB virus-infected, EBNA-negative B-cell populations in IM blood is discussed in terms of the type of infection established by EB virus in the circulation of IM patients.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-38-3-449
1978-03-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/38/3/JV0380030449.html?itemId=/content/journal/jgv/10.1099/0022-1317-38-3-449&mimeType=html&fmt=ahah

References

  1. Baringer J. R. 1974; Recovery of herpes simplex virus from human sacral ganglions. New England Journal of Medicine 291:828–830
    [Google Scholar]
  2. Bastian F. O., Rabson A. S., Yee C. L., Tralka T. S. 1972; Herpesvirus hominis: isolation from human trigeminal ganglia. Science 178:306–307
    [Google Scholar]
  3. Bastian F. O., Rabson A. S., Yee C. L., Tralka T. S. 1974; Herpesvirus varicellae isolated from human dorsal root ganglia. Archives of Pathology 97:331–333
    [Google Scholar]
  4. Boyum A. 1968; Separation of leucocytes from blood and bone marrow. Scandinavian Journal of Clinical and Laboratory Investigation 21: (suppl. 97) 77–89
    [Google Scholar]
  5. Denman M. A., Pelton B. K. 1973; Cell separation by size. In Methodological Developments in Biochemistry pp 185–199 Edited by Reid E. London: Longman;
    [Google Scholar]
  6. Denman M. A., Pelton B. K. 1974; Control mechanisms in infectious mononucleosis. Clinical and Experimental Immunology 18:13–25
    [Google Scholar]
  7. Diehl V., Henle G., Henle W., Kohn G. 1968; Demonstration of a Herpes group virus in cultures of peripheral leucocytes from patients with infectious mononucleosis. Journal of Virology 2:663–669
    [Google Scholar]
  8. Downey H., Mckinlay C. A. 1923; Acute lymphadenosis compared with acute lymphatic leukaemia. Archives of Internal Medicine 32:82–112
    [Google Scholar]
  9. Enberg R. N., Eberle B. J., Williams R. C. 1974; T- and B-cells in peripheral blood during infectious mononucleosis. Journal of Infectious Diseases 130:104–111
    [Google Scholar]
  10. Epstein M. A., Achong B. G. 1973a; Various forms of Epstein–Barr virus infection in man: established facts and a general concept. Lancet ii:836–839
    [Google Scholar]
  11. Epstein M. A., Achong B. G. 1973b; The EB virus. Annual Review of Microbiology 27:413–436
    [Google Scholar]
  12. Finch S. C. 1969; Laboratory findings in infectious mononucleosis. In Infectious Mononucleosis pp 47–62 Edited by Carter R. L., Penman H. G. Oxford: Blackwell;
    [Google Scholar]
  13. Froland S., Natvig J. B., Berdal P. 1971; Surface-bound immunoglobulin as a marker of B lymphocytes in man. Nature New Biology 234:251–252
    [Google Scholar]
  14. Gerber P., Hoyer B. H. 1971; Induction of cellular DNA synthesis in human leucocytes by Epstein–Barr virus. Nature, London 231:46–47
    [Google Scholar]
  15. Greaves M. F., Brown G. 1974; Purification of human T and B lymphocytes. Journal of Immunology 112:420–423
    [Google Scholar]
  16. Greaves F. M., Brown G., Rickinson A. B. 1974; Epstein–Barr virus binding sites on lymphocyte subpopulations and the origin of lymphoblasts in cultured lymphoid cell lines and in the blood of patients with infectious mononucleosis. Clinical Immunology and Immunopathology 3:514–524
    [Google Scholar]
  17. Henle G., Henle W. 1966; Iminunofluorescer.ee in cells derived from Burkitt’s lymphoma. Journal of Bacteriology 91:1248–1256
    [Google Scholar]
  18. Henle G., Henle W., Diehl V. 1968; Relation of Burkitt’s tumour-associated herpes-type virus to infectious mononucleosis. Proceedings of the National Academy of Sciences of the United States of America 59:94–101
    [Google Scholar]
  19. Henle W., Diehl V., Kohn G., Zur Hausen H., Henle G. 1967; Herpes-type virus and chromosome marker in normal leucocytes after growth with irradiated Burkitt cells. Science 157:1064–1065
    [Google Scholar]
  20. Henle W., Henle G. 1973; Evidence for an oncogenic potential of the Epstein–Barr virus. Cancer Research 33:1419–1423
    [Google Scholar]
  21. Jarvis J. E., Ball G., Rickinson A. B., Epstein M. A. 1974; Cytogenetic studies on human lymphoblastoid cell lines from Burkitt’s lymphomas and other sources. International Journal of Cancer 14:716–721
    [Google Scholar]
  22. Jondal M. 1974a; Surface markers on human B and T lymphocytes. IV. Distribution of surface markers on resting and blast-transformed lymphocytes. Scandinavian Journal of Immunology 3:739–747
    [Google Scholar]
  23. Jondal M. 1974b; Surface markers on human B and T lymphocytes. III. A marker for lymphocyte adherence. Scandinavian Journal of Immunology 3:269–276
    [Google Scholar]
  24. Jondal M., Klein G. 1973; Surface markers on human B and T lymphocytes. II. Presence of Epstein–Barr virus (EBV) receptors on B lymphocytes. Journal of Experimental Medicine 138:1365–1378
    [Google Scholar]
  25. Jondal M., Klein G., Oldstone M. B. A., Bokish V., Yefenof E. 1976; Surface markers on human B and T lymphocytes. VIII. The association between complement and Epstein–Barr virus receptors on human lymphoid cells. Scandinavian Journal of Immunology 5:402–410
    [Google Scholar]
  26. Klein G. 1973–74; Immunological surveillance against neoplasia. The Harvey Lectures Series 69: pp 71–102
    [Google Scholar]
  27. Klein G. 1975; The Epstein–Barr virus and neoplasia. New England Journal of Medicine 293:1353–1357
    [Google Scholar]
  28. Klein G., Giovanella B., Westman A., Stehlin J. S., Munford D. 1975; An EBV-genome-negative cell line established from an American Burkitt lymphoma: receptor characteristics, EBV infectability and permanent conversion into EBV-positive sub-lines by in vitro infection. Intervirology 5:319–334
    [Google Scholar]
  29. Klein G., Svedmyr E., Jondal M., Persson P. O. 1976; EBV-determined nuclear antigen (EBNA)-positive cells in the peripheral blood of infectious mononucleosis patients. International Journal of Cancer 17:21–26
    [Google Scholar]
  30. Leibold W., Flanagan T. D., Menezes J., Klein G. 1975; Induction of Epstein–Barr virus associated nuclear antigen during in vitro transformation of human lymphoid cells. Journal of the National Cancer Institute 54:65–68
    [Google Scholar]
  31. Miller R. G., Phillips R. A. 1969; Separation of cells by velocity sedimentation. Journal of Cell Physiology 27:191–202
    [Google Scholar]
  32. Moss D. J., Pope J. H. 1975; EBNA production and cell proliferation in adult peripheral leucocytes inoculated with QIMR-WIL strain of EB-virus. International Journal of Cancer 15:503–511
    [Google Scholar]
  33. Olding L. B., Kingsbury D. T., Oldstone M. B. S. 1976; Pathogenesis of cytomegalovirus infection. Distribution of viral products, immune complexes and autoimmunity during latent murine infection. Journal of General Virology 33:267–280
    [Google Scholar]
  34. Papamichael M., Sheldon P. J., Holborow E. J. 1974; T- and B-cell subpopulations in infectious mononucleosis. Clinical and Experimental Immunology 18:1–11
    [Google Scholar]
  35. Pattengale P. K., Smith R. W., Gerber P. 1973; Selective transformation of B lymphocytes by EB virus. Lancet ii:93–94
    [Google Scholar]
  36. Pope J. H. 1967; Establishment of cell lines from peripheral leucocytes in infectious mononucleosis. Nature, London 216:810–811
    [Google Scholar]
  37. Pope J. H., Horne M. K., Scott W. 1968; Transformation of foetal human leucocytes in vitro by filtrates of a human leukaemic cell line containing herpes-like virus. International Journal of Cancer 3:857–866
    [Google Scholar]
  38. Pope J. H., Scott W., Moss D. J. 1973; Transformation of human lymphoid cells by Epstein–Barr virus involves a special cell population and co-operation between cells. Nature New Biology 246:140–141
    [Google Scholar]
  39. Pulvertaft R. J. V. 1964; Cytology of Burkitt’s tumour (African lymphoma). Lancet 1:238–240
    [Google Scholar]
  40. Reedman B., Klein G. 1973; Cellular localisation of an Epstein–Barr virus (EBV)-associated complementfixing antigen in producer and non-producer lymphoblastoid cell lines. International Journal of Cancer 11:499–520
    [Google Scholar]
  41. Rickinson A. B., Crawford D. H., Epstein M. A. 1977; Inhibition of the in vitro outgrowth of Epstein–Barr virus-transformed lymphocytes by thymus-dependent lymphocytes from infectious mononucleosis patients. Clinical and Experimental Immunology 28:72–79
    [Google Scholar]
  42. Rickinson A. B., Epstein M. A., Crawford D. H. 1975; Absence of infectious Epstein–Barr virus in blood in acute infectious mononucleosis. Nature, London 258:236–238
    [Google Scholar]
  43. Rickinson A. B., Finerty S., Epstein M. A. 1977; Comparative studies on adult donor lymphocytes infected by EB virus in vivo and in vitro: origin of transformed cells arising in co-cultures with foetal lymphocytes. International Journal of Cancer 19:775–782
    [Google Scholar]
  44. Rickinson A. B., Jarvis J. E., Crawford D. H., Epstein M. A. 1974; Observations on the type of infection by Epstein–Barr virus in peripheral lymphoid cells of patients with infectious mononucleosis. International Journal of Cancer 14:704–715
    [Google Scholar]
  45. Rickinson A. B., Jarvis J. E., Crawford D. H., Epstein M. A. 1975; Observations on the nature of Epstein–Barr virus infection of peripheral lymphoid cells in infectious mononucleosis. In Oncogenesis and Herpesviruses III ScientificIARC Scientific Publications No 11 part 2 pp 169–177 Edited by de The G., Epstein M. A., zur Hausen H. Lyon: IARC;
    [Google Scholar]
  46. Royston I., Sullivan J. L., Penman P. O., Perlin E. 1975; Cell-mediated immunity to Epstein–Barr-virus-transformed lymphoblastoid cells in acute infectious mononucleosis. New England Journal of Medicine 293:1159–1163
    [Google Scholar]
  47. Sheldon P. J., Papamichael M., Hemsted E. H., Holborow E. J. 1973; Thymic origin of atypical lymphoid cells in infectious mononucleosis. Lancet 1:1153–1155
    [Google Scholar]
  48. Svedmyr E., Jondal M. 1975; Cytotoxic effector cells specific for B cell lines transformed by Epstein–Barr virus are present in patients with infectious mononucleosis. Proceedings of the National Academy of Sciences of the United States of America 72:1622–1626
    [Google Scholar]
  49. Wybran J., Chantler S., Fudenberg H. H. 1973; Isolation of normal T cells in chronic lymphatic leukaemia. Lancet 1:126–129
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-38-3-449
Loading
/content/journal/jgv/10.1099/0022-1317-38-3-449
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error