1887

Abstract

SUMMARY

This study investigated the synthesis of membrane antigen (MA) as well as virus capsid antigen (VCA) and early antigen (EA) in Daudi cells which had been superinfected with the P3HR-1 strain of Epstein-Barr virus (EBV) and then treated with trypsin to remove initially absorbed MA-positive material from the cell surface. Synthesis of MA, VCA and EA was completely inhibited by puromycin. A marked reduction in the frequency of MA positive cells was observed in superinfected cells cultured in the presence of either cytosine arabinoside (Ara-C) or phosphonoacetate (PA); however, a small fraction of MA synthesis occurred, suggesting an inhibitor insensitive component in MA. A differential absorption of EBV antibody-positive human serum with the Ara-C treated or untreated infected cells detected two antigenically different components in MA: early (Ara-C insensitive) and late (Ara-C sensitive) MA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-38-1-111
1978-01-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/38/1/JV0380010111.html?itemId=/content/journal/jgv/10.1099/0022-1317-38-1-111&mimeType=html&fmt=ahah

References

  1. Dölken G., Klein G. 1976; Expression of Epstein–Barr virus-associated membrane antigen in Raji cells superinfected with two different virus strains. Virology 70:210–213
    [Google Scholar]
  2. Ernberg I., Klein G., Kourilsky F. M., Silvestre D. 1974; Differentiation between early and late membrane antigen on human lymphoblastoid cell lines infected with Epstein–Barr virus. I. Immunofluorescence. Journal of the National Cancer Institute 53:61–65
    [Google Scholar]
  3. Gergely L., Klein G., Ernberg I. 1971; Appearance of Epstein–Barr virus-associated antigens in infected Raji cells. Virology 45:10–21
    [Google Scholar]
  4. Henle G., Henle W. 1966; Immunofluorescence in cells derived from Burkitt’s lymphoma. Journal of Bacteriology 91:1248–1256
    [Google Scholar]
  5. Henle W., Henle G., Zajac B. A., Pearson G., Waubke R., Scriba M. 1970; Differential reactivity of human sera with early antigens induced by Epstein–Barr virus. Science 169:188–190
    [Google Scholar]
  6. Hinuma Y., Konn M., Yamaguchi J., Wudarski D. J., Blakeslee J. R. Jun, Grace J. T. Jun 1967; Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. Journal of Virology 1:1045–1051
    [Google Scholar]
  7. Hinuma Y., Sairenji T., Ohota-Hatano R. 1970; Detection of antibody to a new antigen induced by Epstein–Barr virus in serums from patients with malignant lymphoid diseases. Proceedings of the Japan Academy 46:989–992
    [Google Scholar]
  8. Ida S., Sairenji T., Hinuma Y. 1972; Antibodies to a new antigen induced by Epstein–Barr virus in patients with nasopharyngeal carcinoma, leukemia, malignant lymphoma, and in control groups. Gann 63:49–55
    [Google Scholar]
  9. Klein G., Clifford P., Klein E, Stjernsward J. 1966; Search for tumor-specific immune reactions in Burkitt lymphoma patients by the membrane immunofluorescence reaction. Proceedings of the National Academy of Sciences of the United States of America 55:1628–1635
    [Google Scholar]
  10. Klein G., Dombos L., Gothoskar B. 1972; Sensitivity of Epstein–Barr virus (EBV) producer and nonproducer human lymphoblastoid cell lines to superinfection with EB virus. International Journal of Cancer 10:44–57
    [Google Scholar]
  11. Klein G., Pearson G., Henle G., Henle W., Goldstein G., Clifford P. 1969; Relation between Epstein–Barr viral and cell membrane immunofluorescence in Burkitt tumor cells. III. Comparison of blocking of direct membrane immunofluorescence and anti-EBV reactivities of different sera. Journal of Experimental Medicine 129:697–705
    [Google Scholar]
  12. Klein G., Pearson G., Nadkarni J. S., Nadkarni J. J., Klein E., Henle G., Henle W., Clifford P. 1968; Relation between Epstein–Barr viral and cell membrane immunofluorescence of Burkitt tumor cells. I. Dependence of cell membrane immunofluorescence on presence of EB virus. Journal of Experimental Medicine 128:1011–1020
    [Google Scholar]
  13. Nadkarni J. S., Nadkarni J. J., Clifford P., Monolov G., Fenyo E. M., Klein E. 1969; Characteristics of new cell lines derived from Burkitt lymphomas. Cancer 23:64–79
    [Google Scholar]
  14. Nagoya T., Hinuma Y. 1972; Production of infective Epstein–Barr virus in a Burkitt lymphoma cell line, P3HR-1. Gann 63:87–93
    [Google Scholar]
  15. Nonoyama M., Pagano J. S. 1971; Detection of Epstein–Barr viral genome in non-productive cells. Nature New Biology 233:103–106
    [Google Scholar]
  16. Pearson G. R., Henle G., Henle W. 1971; Production of antigens associated with Epstein–Barr virus in experimentally infected lymphoblastoid cell lines. Journal of the National Cancer Institute 46:1243–1250
    [Google Scholar]
  17. Reedman B., Klein G. 1973; Cellular localization of an Epstein–Barr virus (EBV)-associated complementfixing antigen in producer and nonproducer lymphoblastoid cell lines. International Journal of Cancer 11:499–520
    [Google Scholar]
  18. Sairenji T., Hinuma Y. 1973; Modes of Epstein–Barr virus-infection in human floating cell lines. Gann 64:583–590
    [Google Scholar]
  19. Sairenji T., Hinuma Y. 1975; Ultraviolet inactivation of Epstein–Barr virus: Effect on synthesis of virus-associated antigens. International Journal of Cancer 16:1–6
    [Google Scholar]
  20. Silvestre D., Ernberg I., Neauport-Sautes C., Kourilsky F. M., Klein G. 1974; Differentiation between early and late membrane antigen on human lymphoblastoid cell lines infected with Epstein–Barr virus. II. Immunoelectron microscopy. Journal of the National Cancer Institute 53:67–74
    [Google Scholar]
  21. Svedmyr A., Demissie A., Klein G., Clifford P. 1970; Antibody patterns in different human sera against intracellular and membrane-antigen complex associated with Epstein–Barr virus. Journal of the National Cancer Institute 44:595–610
    [Google Scholar]
  22. Takahashi H., Hinuma Y. 1970; Nature of antigens of cultured Burkitt lymphoma cells, as revealed by membrane immunofluorescence. Gann 61:337–346
    [Google Scholar]
  23. Thorley-Lawson D., Strominger J. L. 1976; Transformation of human lymphocytes by Epstein–Barr virus is inhibited by phosphonoacetic acid. Nature, London 263:332–334
    [Google Scholar]
  24. Yajima Y., Nonoyama M. 1976; Mechanisms of infection with Epstein–Barr virus. I. Viral DNA replication and formation of noninfectious virus particles in superinfected Raji cells. Journal of Virology 19:187–194
    [Google Scholar]
  25. Yajima Y., Tanaka A., Nonoyama M. 1976; Inhibition of productive replication of Epstein–Barr virus DNA by phosphonoacetic acid. Virology 71:352–354
    [Google Scholar]
  26. Zur Hausen H., Henle W., Hummeler K., Diehl V., Henle G. 1967; Comparative study of cultured Burkitt tumor cells by immunofluorescence, autoradiography, and electron microscopy. Journal of Virology 1:830–837
    [Google Scholar]
  27. Zur Hausen H., Schulte-Holthausen H. 1970; Presence of EB virus nucleic acid homology in a ‘virus-free, line of Burkitt tumor cells. Nature, London 227:245–248
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-38-1-111
Loading
/content/journal/jgv/10.1099/0022-1317-38-1-111
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error