1887

Abstract

SUMMARY

Factors that control focus morphology in cell cultures infected with Bryan strain Rous sarcoma virus (B-RSV) were examined. Cloning experiments with the roundc-cell (morph) and fusiform cell (morph) variants of RSV (Temin, 1960), confirmed that the two mutant viruses breed true and that these two distinct focus types were under the control of the virus genome.

Three subvariants of the morph focus type were described which failed to breed true in cloning experiments. It was concluded that this type of focus variation was not due to virus mutation.

Variation in focus morphology was observed when RSV was plated on chick embryo fibroblast cell lines from different sources, as reflected in the relative frequency of expression of the three morph focus types. These variations were exhibited between fibroblast cell lines of different genetic background, indicating that the cell genome had an influence on focus expression. Focus expression also varied in fibroblast cell lines from chickens of different ages. The strongest host influence on focus expression was observed with fibroblast cell lines derived from selected individual tissue of single embryos. The latter two factors (age and tissue origin) suggested that the differentiated state of the cells also influences RSV focus morphology.

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1977-09-01
2022-01-20
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References

  1. Dougherty R. M., Rasmussen R. 1964; Properties of a strain of Rous sarcoma virus that infects mammals. National Cancer Institute Monograph 17:337–350
    [Google Scholar]
  2. Dougherty R. M., Simons P. J. 1962; The sensitivity of Rous sarcoma virus assays. Virology 18:559–566
    [Google Scholar]
  3. Dougherty R. M., Simons P. J., Chesterman F. C. 1963; Biological properties of three variants of Rous sarcoma virus. Journal of the National Cancer Institute 31:1285–1307
    [Google Scholar]
  4. Duff R. G., Vogt P. K. 1969; Characteristics of two new avian tumor virus subgroups. Virology 39:18–30
    [Google Scholar]
  5. Hanafusa H. 1965; Analysis of the defectiveness of Rous sarcoma virus. III. Determining influence of a new helper virus on the host range and susceptibility to interference of RSV. Virology 25:248–255
    [Google Scholar]
  6. Hanafusa H., Hanafusa T., Rubin H. 1963; The defectiveness of Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 49:572–580
    [Google Scholar]
  7. Ishizaki R., Vogt P. K. 1966; Immunological relationships among envelope antigens of avian tumor viruses. Virology 30:375–387
    [Google Scholar]
  8. Rubin H. 1960; A virus in chick embryos which induces resistance in vitro to Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 46:1105–1119
    [Google Scholar]
  9. Rubin H. 1964; Virus defectiveness and cell transformation in the Rous sarcoma. Journal of Cellular and Comparative Physiology 64:173–179
    [Google Scholar]
  10. Rubin H., Vogt P. K. 1962; An avian leukosis virus associated with stocks of Rous sarcoma virus. Virology 17:184–194
    [Google Scholar]
  11. Sarma P. S., Huebner R. J., Armstrong P. 1964; A simplified tissue culture tube neutralization test for Rous sarcoma antibodies. Proceedings of the Society for Experimental Biology and Medicine 115:471–486
    [Google Scholar]
  12. Siminoff P., Reed F. C. 1963; A macroscopic method for counting foci produced by Rous sarcoma virus in vitro. Virology 21:284–285
    [Google Scholar]
  13. Temin H. M. 1960; The control of cellular morphology in embryonic cells infected with Rous sarcoma virus in vitro. Virology 10:182–197
    [Google Scholar]
  14. Temin H. M., Rubin H. 1958; Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in culture. Virology 6:669–688
    [Google Scholar]
  15. Vogt P. K. 1965; Avian tumor viruses. Advances in Virus Research 11:293–385
    [Google Scholar]
  16. Vogt P. K., Ishizaki R. 1965; Reciprocal patterns of genetic resistance to avian tumor viruses in two lines of chickens. Virology 26:664–672
    [Google Scholar]
  17. Vogt P. K., Ishizaki R. 1966; Patterns of viral interference in the avian leukosis and sarcoma complex. Virology 30:368–374
    [Google Scholar]
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