1887

Abstract

SUMMARY

The nuclear polyhedrosis viruses of and produce plaques in monolayers of cells under a solid overlay containing agarose as the solidifying agent. Plaques are visible macroscopically after staining the cells with neutral red or with the tetrazolium salt, INT, and a linear dose response is observed. The sensitivity of the assay is less than that obtained using an end-point dilution technique; however, plaque formation does provide a simple means of cloning virus.

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/content/journal/jgv/10.1099/0022-1317-36-2-361
1977-08-01
2022-01-17
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