1887

Abstract

SUMMARY

The antiviral factor (AVF) from virus-infected plants, purified on polyacrylamide gels, could be labelled with radioactive phosphorus and its activity could be eluted from the gels. The radioactivity and the antiviral activity were co-purified and thus co-electrophoresed; hence, the previously reported radioactive zone (Antignus, Sela & Harpaz, 1975) can be regarded as AVF. The production of AVF requires both the presence of the N-gene in the plant as well as virus infection. AVF production is inhibited by actinomycin D, but its activity is not affected by this drug. AVF production is correlated in time with the development of virus resistance in a local-lesion host. AVF inhibits TMV multiplication in infected leaves and suppresses virus synthesis almost totally in a local-lesion host. Some AVF is also produced when L. is infected with a non-localized virus, but to a much lesser extent and at a later stage of infection. The production of AVF in is not blocked at 30 °C, even though TMV is no longer localized at this temperature.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-35-1-107
1977-04-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/35/1/JV0350010107.html?itemId=/content/journal/jgv/10.1099/0022-1317-35-1-107&mimeType=html&fmt=ahah

References

  1. Antignus Y., Sela I., Harpaz I. 1975; A phosphorus-containing fraction associated with antiviral activity in Nicotiana spp. carrying the gene for localization of TMV infection. Physiological Plant Pathology 6:159–168
    [Google Scholar]
  2. Gianinazzi S., Kassanis B. 1974; Virus resistance induced in plants by polyacrylic acid. Journal of General Virology 23:1–9
    [Google Scholar]
  3. Jockusch H. 1966; The role of host genes, temperature and polyphenoloxidase in the necrotization of TMV infected tobacco tissue. Phytopathologische Zeitschrift 55:185–192
    [Google Scholar]
  4. Kado C. L., Knight C. A. 1968; Location of a local lesion gene in tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences of the United States of America 55:1276–1283
    [Google Scholar]
  5. Kassanis B., White R. F. 1974; Inhibition of acquired resistance to tobacco mosaic virus by actinomycin D. Journal of General Virology 25:323–324
    [Google Scholar]
  6. Loebenstein G. 1972; Localization and induced resistance in virus infected plants. Annual Review of Phytopathology 10:177–200
    [Google Scholar]
  7. Loebenstein G., Sela B., Van Praagh T. 1969; Increase of tobacco mosaic local lesion size and virus multiplication in hypersensitive hosts in the presence of actinomycin D. Virology 37:42–48
    [Google Scholar]
  8. Otsuki Y., Shimomura T., Takebe I. 1972; Tobacco mosaic virus multiplication and expression of the N-gene in necrotic responding tobacco varieties. Virology 50:45–50
    [Google Scholar]
  9. Sänger H. L., Knight C. A. 1963; Action of actinomycin D on RNA synthesis in healthy and virus-infected tobacco leaves. Biochemical and Biophysical Research Communications 13:455–461
    [Google Scholar]
  10. Sela L., Applebaum S. W. 1962; Occurrence of an antiviral factor in virus-infected plants. Virology 17:543–548
    [Google Scholar]
  11. Sela I., Harpaz I., Birk Y. 1964; Separation of a highly active antiviral factor from virus-infected plants. Virology 22:446–451
    [Google Scholar]
  12. Van Loon L. C., Van Kammen A. 1970; Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var‘Samsun NN’ II. Changes in protein constitution after infection with tobacco mosaic virus. Virology 40:199–211
    [Google Scholar]
  13. Weintraub M., Kemp M. W. J., Ragetli H. W. J. 1961; Some observations on hypersensitivity to plant viruses. Phytopathology 51:290–293
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-35-1-107
Loading
/content/journal/jgv/10.1099/0022-1317-35-1-107
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error